In vitro antibacterial effects of the dentin primer of Clearfil Protect Bond
In vitro antibacterial effects of the dentin primer of Clearfil Protect Bond
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DOI：
10.1016/j.dental.2005.05.009
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发表时间：
2006-06-01
期刊：
影响因子：
5
通讯作者：
Peters, MC
中科院分区：
文献类型：
--
作者：
Imazato, S;Kuramoto, A;Peters, MC
目标。本研究旨在研究市售自酸蚀粘合剂系统 Clearfil Protect Bond 的牙本质底漆的抗菌效果，该系统含有抗菌单体 12-甲基丙烯酰氧基十二烷基溴化吡啶 (MDPB)。使用含有 5% MDPB 和酸性粘附促进单体 MDP 的 Clearfil Protect Bond 底漆、仅含有 MDP 的底漆和含有 1% 氯化十六烷基吡啶的底漆，通过琼脂盘扩散法检查对变形链球菌、干酪乳杆菌或内氏放线菌的抑制效果。通过连续微量稀释测定确定每种引物对三种细菌物种的最低抑制/杀菌浓度（MIC/MBC）。为了测试牙本质中的杀菌效果，将底漆应用于已浸渍变形链球菌的脱矿牙本质块上，并对活细菌的数量进行计数。 结果。对于所有三种细菌，Clearfil Protect Bond 引物产生的抑菌圈大小明显大于其他引物 (p
Objectives. This study aimed to investigate the antibacterial effects of the dentin primer of a commercially available self-etching adhesive system, Clearfil Protect Bond, which contains antibacterial monomer 12-methacrytoyloxydodecyl-pyridinium bromide (MDPB).Methods. Inhibitory effects against Streptococcus mutans, Lactobacillus casei, or Actinomyces naeslundii were examined by an agar-disc diffusion method using the Clearfil Protect Bond primer containing 5% MDPB and an acidic adhesion-promoting monomer MDP, the primer only with MDP, and the primer with 1% cetylpyridinium chloride. The minimum inhibitory/bactericidal concentrations (MIC/MBC) of each primer for the three bacterial, species were determined by serial microdilution assays. For testing the bactericidal effects seen in dentin, the primer was applied to demineralized dentin blocks in which S. mutans had been impregnated, and numbers of viable bacteria were counted.Results. For all three bacteria, the sizes of the inhibition zones produced by Clearfil Protect Bond primer were significantly greater than for the other primers (p