Gene expression signature associated with BRAF mutations in human primary cutaneous melanomas
Gene expression signature associated with BRAF mutations in human primary cutaneous melanomas
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DOI：
10.1016/j.molonc.2008.01.002
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发表时间：
2008-04-01
影响因子：
6.6
通讯作者：
Bressac-de Paillerets, Brigitte
中科院分区：
文献类型：
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作者：
Kannengiesser, Caroline;Spatz, Alain;Bressac-de Paillerets, Brigitte
为了将BRAF突变状态与人类原发性皮肤黑色素瘤中的基因表达联系起来，从而更深入地了解BRAF突变对细胞生物学的影响，我们分析了从用于表达研究的相同组织载玻片中提取DNA的黑色素瘤中获得的所有表达数据。对69例冷冻原发性黑色素瘤患者进行BRAF和NRAS基因分型，这些患者的寡核苷酸微阵列表达数据可用。根据BRAF突变状态重新分析这些黑色素瘤的表达数据。一组250个探针，代表209个基因，显著(原P
With the aim to correlate BRAF mutation status with gene expression in human primary cutaneous melanomas, and thus to get more insight on the consequences of BRAF mutation on cell biology, we analyzed all expression data obtained in melanomas from which DNA was extracted from the same tissue slides that were used for the expression study. A cohort of 69 frozen primary melanoma whose oligonucleotide micro-array expression data were available, were genotyped for BRAF and NRAS genes. The expression data from these melanomas were re-analyzed according to BRAF mutational status.A set of 250 probes representing 209 genes that were significantly (raw P