Characterization of Chinese hamster ovary cell culture feed media precipitate
Characterization of Chinese hamster ovary cell culture feed media precipitate
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DOI：
10.1002/btpr.3188
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发表时间：
2021-07-02
影响因子：
2.9
通讯作者：
Yoon, Seongkyu
中科院分区：
文献类型：
--
作者：
Hoang, Duc;Galbraith, Shaun;Yoon, Seongkyu
单克隆抗体生产的工艺强化导致补料培养基浓度更高，从而导致培养基溶液中固体沉淀的问题。这会导致处理问题，因为沉淀物中的成分不再处于溶液中，从而改变了培养基成分并导致细胞培养性能的变化。这项工作的目标是表征进料介质沉淀物，特别是识别沉淀成分，以便制定缓解策略。根据进行的分析，发现沉淀物主要是有机的，并通过液相色谱-质谱法和电感耦合等离子体发射光谱法（ICP-OES）进行分析，以鉴定其组成成分。鉴定出多达 10 种氨基酸，其中酪氨酸（约占 77 wt.%）和苯丙氨酸（约 4 wt.%）是最常见的氨基酸。 ICP-OES 的元素分析表明，无机组分占固体沉淀物的重量百分比不到一％，其中金属硫酸盐是主要的无机组分。
Process intensification of monoclonal antibody production is leading to more concentrated feed media causing issues with precipitation of solids from the media solution. This results in processing problems since components in the precipitate are no longer in solution, changing the media composition and leading to variability in cell culture performance. The goal of this work is to characterize the feed media precipitate, and in particular to identify the precipitated components so that mitigation strategies can be developed. From the conducted analysis, the precipitate was predominately found to be organic and was analyzed with liquid chromatography-mass spectrometry and inductively coupled plasma-optical emission spectroscopy (ICP-OES) to identify the constituent components. Up to ten amino acids were identified with tyrosine (approximately 77 wt.%) and phenylalanine (approximately 4 wt.%) being the most prevalent amino acids. Elemental analysis with ICP-OES revealed that inorganic components were accounted for less than one weight percentage of the solid precipitate with metal sulfates being the predominant inorganic components.