Quantitative Differences in TGF-β Family Members Measured in Small Antral Follicle Fluids From Women With or Without PCO
Quantitative Differences in TGF-β Family Members Measured in Small Antral Follicle Fluids From Women With or Without PCO
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DOI：
10.1210/jc.2019-01094
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发表时间：
2019-12-01
影响因子：
5.8
通讯作者：
Andersen, Claus Yding
中科院分区：
文献类型：
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作者：
Kristensen, Stine Gry;Kumar, Ajay;Andersen, Claus Yding
内容：TGF-β家族成员与多囊卵巢（PCO）妇女的异常卵泡发育有关。目的：探讨多囊卵巢妇女和非多囊卵巢妇女的小窦卵泡（hSAFs）液中TGF-β家族成员的浓度是否存在定量差异。设计和设置：从接受卵巢组织冷冻保存的妇女获得的4- 11-mm hSAFs中收集卵泡液（FF），患者：16名患有PCO的妇女（FF = 93）和33名没有PCO的妇女（FF = 92）。总胆红素;激活素-A、激活素-B和激活素-AB;卵泡抑素;卵泡抑素样-3;雌二醇;和睾酮。活化素B与其他生长因子有显著相关性。FFS的浓度与PCO的妇女，特别是在hSAFs 6 mm的HSP-A和HSP-B的浓度显着降低。结论：改变浓度的TGF-β家族成员在hSAFs从PCO的妇女突出改变生长因子信号作为一个潜在的机制卵泡生长停滞。
Context: Members of the TGF-beta family have been implicated in aberrant follicle development in women with polycystic ovaries (PCO).Objective: Are there quantitative differences in the concentrations of TGF-beta family members in fluid from human small antral follicles (hSAFs) in women with or without PCO?Design and Setting: Follicle fluids (FFs) were collected from 4- to 11-mm hSAFs obtained from women undergoing ovarian tissue cryopreservation for fertility preservation.Patients: FFs from 16 women with PCO (FF = 93) and 33 women without PCO (FF = 92).Main Outcome Measures: Intrafollicular concentrations of growth differentiation factor-9 (GDF9); anti-Mullerian hormone (AMH); inhibin-A and inhibin-B; total inhibin; activin-A, activin-B, and activin-AB; follistatin; follistatin-like-3; estradiol; and testosterone.Results: Activin-B concentrations were reported in hSAFs, and concentrations were 10 times higher than activin-A and activin-AB concentrations. Activin-B showed significant associations with other growth factors. Concentrations of inhibin-A and inhibin-B were significantly lower in FFs from women with PCO, especially in hSAFs 6 mm.Conclusions: Altered concentrations of TGF-beta family members in hSAFs from women with PCO highlight altered growth factor signaling as a potential mechanism for follicle growth arrest.