Effects of naloxone on the serum luteinizing hormone level and the number of Fos-positive gonadotropin-releasing hormone neurons in immature female rats
Effects of naloxone on the serum luteinizing hormone level and the number of Fos-positive gonadotropin-releasing hormone neurons in immature female rats
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DOI：
10.1016/s0006-8993(99)02447-6
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发表时间：
2000-03-06
期刊：
影响因子：
2.9
通讯作者：
Kimura, F
中科院分区：
文献类型：
--
作者：
He, DQ;Mitsushima, D;Kimura, F
为探讨阿片受体拮抗剂对雌性大鼠促性腺激素释放激素(GnRH)神经元数量发育的影响，对未成熟雌性大鼠(出生后16天和30天)和成年雌性大鼠注射纳洛酮(NAL)后，采血并进行Fos和GnRH免疫细胞化学双标分析。3个年龄组大鼠在皮下注射NAL(2.5 mg/kg)或生理盐水90min后，注入4%多聚甲醛-PB。同时进行组织孵育和双标免疫细胞化学染色。虽然GnRH神经元的总数没有明显的发育变化(p>；0.05)，但NAL诱导的血清黄体生成素(LH)浓度在d16组明显高于d30组和成熟组(p<；0.01)。相反，在d16，Fos阳性的GnRH神经元很少，在d30组(p<；0.05比生理盐水)和成熟组(p<；0.01比生理盐水)观察到一些Fos阳性的GnRH神经元。这些结果提示，阿片能抑制系统对幼年雌性大鼠的GnRH神经元的抑制作用与成熟雌性大鼠不同。(C)2000 Elsevier Science B.V.保留所有权利。
To examine developmental changes in the number of gonadotropin-releasing hormone (GnRH) neurons activated by an opioid receptor antagonist in female rats, blood sampling and double-labeled immunocytochemistry for Fos and GnRH were performed after the injection of naloxone (NAL) in immature (postnatal d16 and d30) and mature female rats. Three age groups of rats were perfused with 4% paraformaldehyde-PB 90 min after the subcutaneous injection of NAL (2.5 mg/kg) or saline. All tissue incubation and staining for double-labeled immunocytochemistry were simultaneously performed. Although no significant developmental change was observed in the total number of GnRH neurons (p > 0.05), NAL-induced increases in serum luteinizing hormone (LH) concentrations were much greater in the d16 group than those in the d30 and mature groups (p < 0.01). Conversely, Fos-positive GnRH neurons were rarely observed in d16, and some Fos-positive GnRH neurons were observed in the d30 group (p < 0.05 vs. saline) and the mature group (p < 0.01 vs. saline). These results suggest that opiatergic inhibitory system on GnRH neuron in immature female rats is different from that in mature female rats. (C) 2000 Elsevier Science B.V. All rights reserved.