Sucrose signaling function on the formation and swelling of bulblets of Lilium sargentiae EH Wilson
Sucrose signaling function on the formation and swelling of bulblets of Lilium sargentiae EH Wilson
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DOI：
10.1007/s11240-018-1451-4
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发表时间：
2018-10-01
影响因子：
3
通讯作者：
Si, Wenhui
中科院分区：
文献类型：
--
作者：
Gao, Suping;Zhu, Yuan;Si, Wenhui
大羽百合威尔逊是百合科（Liliaceae）的一种观赏和经济的开花鳞茎植物。不幸的是，由于球茎自然繁殖率低或幼年期较长，新基因型的商业释放可能需要10年甚至20年的时间。在这项研究中，通过添加蔗糖在试管中扩大鳞茎，以缩短商业生产周期的持续时间。结果表明，在培养基中添加60 gl（-1）蔗糖时，小鳞茎的诱导和膨大效果最好。研究了蔗糖对L.红藤明显占优势。这与蔗糖的渗透势无关，也不能完全用蔗糖作为能量和碳源的功能来解释。本研究利用蔗糖类似物对小鳞茎进行诱导培养，研究蔗糖特异性信号对小鳞茎形成和扩展的影响。结果表明，外源3-O-甲基葡萄糖不能诱导试管小鳞茎的形成，其诱导效果与甘露糖相似，而异麦芽酮糖则能诱导试管小鳞茎的形成，其诱导效果与蔗糖相似。结果进一步表明，蔗糖的特异性信号可能是对小鳞茎形成和膨胀的影响的原因。我们采用排除法证实了上述结果和假设，并观察到只有少部分蔗糖被细胞壁转化酶转化，通过己糖转运蛋白进入细胞，通过己糖或己糖信号系统对鳞茎的生长发育起一定的作用。总的来说，蔗糖对小鳞茎诱导和膨胀的影响可以通过蔗糖特异性信号来解释。此外，这种效应与蔗糖特异性载体直接相关。
Lilium sargentiae E.H. Wilson (Liliaceae) is an ornamental and economic flowering bulb in China. Unfortunately, due to low natural bulb multiplication rates or long juvenile phases, commercial release of a new genotype may take 10 or even 20years. In this study, bulbs were expanded in test tubes by adding sucrose, to shorten the duration of the commercial production cycle. Results indicated that when sucrose, with a concentration of 60gl(-1), was supplied to the culture medium, the bulblets could be most effectively induced and expanded. The effect of sucrose on the formation and expansion of L. sargentiae was obvious and dominant. It was not connected to the osmotic potential of sucrose, nor entirely explained by the function of sucrose as an energy and carbon source. We used sugar analogues in the culturing of explanted materials to research the effect of sucrose-specific signaling on bulblet formation and expansion. Results showed that exogenous 3-O-methyl glucose could not induce and expand test-tube bulblets, which was similar to mannose, whereas isomaltulose could generate similar results to those obtained with sucrose. The results further indicated that the specific signalling of sucrose could be responsible for the effect on bulblets formation and swelling. We used the exclusion method to confirm above results and hypothesis, and observed that only a small part of sucrose was transformed by cell wall invertase and entered the cells through hexose transporters, playing a certain effect on bulbelt growth and development through hexose or hexose signaling systems. Overall, the effect of sucrose on bulblet induction and swelling could be explained by sucrose-specific signaling. Furthermore, this effect has a direct correlation with the sucrose-specific vector.