Genome-wide investigation and expression analysis suggest diverse roles of auxin-responsive GH3 genes during development and response to different stimuli in tomato (Solanum lycopersicum)
Genome-wide investigation and expression analysis suggest diverse roles of auxin-responsive GH3 genes during development and response to different stimuli in tomato (Solanum lycopersicum)
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DOI：
10.1007/s00438-011-0672-6
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发表时间：
2012-03-01
影响因子：
3.1
通讯作者：
Sharma, Arun K.
中科院分区：
文献类型：
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作者：
Kumar, Rahul;Agarwal, Priyanka;Sharma, Arun K.
在植物中，生长素介导的反应受多种蛋白质调控。其中一类蛋白质，即GH3，参与吲哚乙酸（IAA）与氨基酸的结合，并提供一个负反馈回路来控制生长素的内稳态。为了更好地理解生长素作用的机制，利用现有的番茄表达序列标签（EST）数据库鉴定出了15个编码GH3成员的基因。它们的直系同源基因从烟草、马铃薯、本氏烟草、辣椒和矮牵牛中被鉴定出来。对拟南芥GH3、番茄GH3及其茄科直系同源基因的系统发育分析为这些蛋白质之间的各种直系同源关系提供了见解。发现这些基因对多种信号有响应，包括植物激素和环境胁迫。对其启动子中生长素响应元件（AuxRE）的分析表明，该元件在序列和数量上存在差异。仅有11个番茄GH3基因在外源生长素作用下上调，这表明AuxRE元件在序列和数量上的多样性与生长素的诱导性之间可能存在关系。对番茄GH3基因在不同营养和生殖组织/阶段的表达分析表明，大多数番茄GH3基因在果实成熟起始阶段作用有限或没有作用。然而，番茄GH3 - 1和 - 2在果实成熟开始时上调，表明这些基因可能在果实成熟中起作用。本研究对番茄的GH3基因家族及其与其他茄科物种和拟南芥中该家族成员的进化关系进行了表征。它有助于鉴定GH3基因，并揭示它们在其他茄科成员的营养/生殖发育阶段的功能。
In plants, auxin-mediated responses are regulated by diverse proteins. One such class of proteins, i.e. GH3, is involved in the conjugation of IAA to amino acids and provides a negative feedback loop to control auxin homoeostasis. In order to have a better understanding of the mechanism of the auxin action, 15 genes encoding GH3 members were identified using existing EST databases of tomato. Their orthologs were identified from tobacco, potato, N. benthemiana, pepper, and petunia. Phylogenetic analysis of AtGH3, SlGH3, and their Solanaceae orthologs provided insights into various orthologous relationships among these proteins. These genes were found to be responsive to a variety of signals including, phytohormones and environmental stresses. Analysis of AuxRE elements in their promoters showed variability in the sequence as well as number of this element. Up-regulation of only 11 SlGH3 genes, in response to exogenous auxin, suggested possible relationship between the diversity in the sequence and number of AuxRE element with the auxin inducibility. Expression analysis of SlGH3 genes in different vegetative and reproductive tissues/stages suggested limited or no role for most of the SlGH3 genes at the initiation of fruit ripening. However, up-regulation of SlGH3-1 and -2 at the onset of fruit ripening indicates that these genes could have a role in fruit ripening. The present study characterizes GH3 gene family of tomato and its evolutionary relationship with members of this family from other Solanaceae species and Arabidopsis. It could help in the identification of GH3 genes and revelation of their function during vegetative/reproductive development stages from other Solanaceae members.