Uptake ability of hepatic sinusoidal endothelial cells and enhancement by lipopolysaccharide
Uptake ability of hepatic sinusoidal endothelial cells and enhancement by lipopolysaccharide
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DOI：
10.2220/biomedres.26.99
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发表时间：
2005-06-01
影响因子：
1.2
通讯作者：
Iwanaga, Toshihiko
中科院分区：
文献类型：
--
作者：
Kamimoto, Miyuki;Rung-Ruangkijkrai, Tilladit;Iwanaga, Toshihiko
肝脏是从血液循环中清除外源性物质和废物的主要器官之一。肝巨噬细胞(Kupffer细胞)和肝窦内皮细胞负责肝脏的清道夫功能。这种被称为清道夫内皮细胞的正弦内皮细胞被认为在正常情况下只吸收可溶性物质和纳米颗粒，而库普弗细胞可以吸收更大的颗粒和整个细胞。然而，在特殊条件下，正弦形内皮细胞可能具有摄取相当大颗粒的潜力。在这项形态学研究中，我们比较了静脉注射直径为20 mm、100 mm和500 nm的乳胶珠、牛血清白蛋白(BSA)和葡聚糖后，肝窦内皮细胞和Kupffer细胞的摄取能力。在正常条件下，正弦形内皮细胞旺盛地吸收100 nm大小的乳胶珠和20 nm大小的乳胶珠。BSA和葡聚糖可被内皮细胞摄取，但不能被库普弗细胞摄取。给予内毒素(LPS)，模拟炎症，刺激内皮细胞摄取。在脂多糖刺激条件下，库普弗细胞对乳胶珠的摄取也增加，但对牛血清白蛋白和葡聚糖的摄取没有增加。这些发现表明，窦状内皮细胞不仅需要摄取可溶性物质，而且需要摄取比预期更大的颗粒，并且在炎症条件下其摄取能力增强。
The liver is one of the major organs that remove exogenous substances and waste products from the blood circulation. Hepatic macrophages (Kupffer cells) and sinusoidal endothelial cells are responsible for the scavenger function of the liver. The sinusoidal endothelial cells, called scavenger endothelial cells, are believed to take up only soluble substances and nanometer-sized particles under normal conditions, while Kupffer cells can ingest larger particles and whole cells. However, the sinusoidal endothelial cells may have the potential to take up considerably large particles under special conditions. In this morphological study, we compared the uptake ability between sinusoidal endothelial cells and Kupffer cells after intravenous injections of latex beads (20 run, 100 rim and 500 nm in diameter), bovine serum albumin (BSA) and dextran. Under normal conditions, the sinusoidal endothelial cells vigorously took up 100-nm-sized latex beads as well as 20-nm latex beads. BSA and dextran were ingested by the endothelial cells but not the Kupffer cells. The administration of lipopolysaccharide (LPS), which mimics Inflammation, stimulated the uptake by endothelial cells. The uptake of latex beads by Kupffer cells was also elevated under LPS-stimulated conditions, but the uptake of BSA and dextran by them was not. These findings suggest that the sinusoidal endothelial cells call ingest not only soluble substances but also larger particles than those expected, and their uptake ability is strengthened under inflammatory conditions.