Clonal spread of antimicrobial-resistant Escherichia coli isolates among pups in two kennels
Clonal spread of antimicrobial-resistant Escherichia coli isolates among pups in two kennels
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DOI：
10.1186/1751-0147-53-11
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发表时间：
2011-02-17
影响因子：
2.1
通讯作者：
Takahashi, Toshio
中科院分区：
文献类型：
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作者：
Harada, Kazuki;Morimoto, Erika;Takahashi, Toshio
尽管养犬业在许多国家很常见，但在狗舍里的幼崽中是否存在抗菌素耐药性细菌的调查还很少。这项研究是为了更好地了解从未经抗菌药物处理的犬舍幼崽中分离到的耐药大肠杆菌的流行病学。我们调查了两个养殖场43只幼犬的86株粪便大肠埃希菌对11种抗菌药的敏感性和超广谱β-内酰胺酶(ESBL)的流行情况。用脉冲场凝胶电泳法(PFGE)分析所有分离株之间的遗传亲缘关系。药敏试验显示，76%的菌株对一种或多种抗菌药耐药，其中对二氢链霉素的耐药率最高(66.3%)，其次为氨苄西林(60.5%)、新诺明-磺胺甲恶唑(41.9%)、土霉素(26.7%)和氯霉素(26.7%)。多药耐药指的是对两种或两种以上抗菌药的耐药性，其中52株(60.5%)耐药。一个养殖场的三只幼崽携带有SHV-12产超广谱β-内酰胺酶分离株。两个养殖场之间的比较表明，对7种抗菌素的抗药性频率和抗药性表型的变异明显不同。PFGE分析表明，两窝同窝幼崽间克隆共享率差异不显著(64.0%vs.88.9%)，而不同窝次幼崽间克隆共享率差异显著(72.0%vs.33.3%，P<；0.05)。两个狗舍中的幼崽都有抗菌素的大肠杆菌克隆，包括耐多药的克隆和产生ESBL的克隆。耐药和敏感细菌很可能会在相同和/或不同的窝中克隆传播，从而影响耐药性流行。
Although the dog breeding industry is common in many countries, the presence of antimicrobial resistant bacteria among pups in kennels has been infrequently investigated. This study was conducted to better understand the epidemiology of antimicrobial-resistant Escherichia coli isolates from kennel pups not treated with antimicrobials. We investigated susceptibilities to 11 antimicrobials, and prevalence of extended-spectrum beta-lactamase (ESBL) in 86 faecal E. coli isolates from 43 pups in two kennels. Genetic relatedness among all isolates was assessed using pulsed-field gel electrophoresis (PFGE). Susceptibility tests revealed that 76% of the isolates were resistant to one or more of tested antimicrobials, with resistance to dihydrostreptomycin most frequently encountered (66.3%) followed by ampicillin (60.5%), trimethoprim-sulfamethoxazole (41.9%), oxytetracycline (26.7%), and chloramphenicol (26.7%). Multidrug resistance, defined as resistance against two or more classes of antimicrobials, was observed in 52 (60.5%) isolates. Three pups in one kennel harboured SHV-12 ESBL-producing isolates. A comparison between the two kennels showed that frequencies of resistance against seven antimicrobials and the variation in resistant phenotypes differed significantly. Analysis by PFGE revealed that clone sharing rates among pups of the same litters were not significantly different in both kennels (64.0% vs. 88.9%), whereas the rates among pups from different litters were significantly different between the two kennels (72.0% vs. 33.3%, P < 0.05). The pups in the two kennels had antimicrobial-resistant E. coli clones, including multidrug-resistant and ESBL-producing clones. It is likely that resistant and susceptible bacteria can clonally spread among the same and/or different litters thus affecting the resistance prevalence.