PARTICIPATION OF ALPHA-ACTININ IN THE CAPPING OF CELL-MEMBRANE COMPONENTS
PARTICIPATION OF ALPHA-ACTININ IN THE CAPPING OF CELL-MEMBRANE COMPONENTS
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DOI：
10.1016/0092-8674(79)90202-2
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发表时间：
1979-01-01
期刊：
影响因子：
64.5
通讯作者：
SINGER, SJ
中科院分区：
文献类型：
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作者：
GEIGER, B;SINGER, SJ
双重荧光染色实验证明细胞内α-辅肌动蛋白在多种配体诱导的几种细胞中在帽和斑下积累。这种现象在用抗H [组蛋白]2血清处理的[小鼠]淋巴细胞和淋巴瘤细胞、用伴刀豆球蛋白A或抗免疫球蛋白抗体处理的脾淋巴细胞和用抗病毒抗原的抗血清处理的VSV[水泡性口炎病毒]感染的小鼠成纤维细胞系MC 57中得到证实。它发生在快速和缓慢的加盖过程中，可以通过直接或间接的配体诱导的再分配。这些观察是在整个细胞上进行的。对于其他细胞骨架蛋白，如细丝蛋白，原肌球蛋白和肌球蛋白，帽下的一个类似的积累是不容易明显的使用全细胞安装，虽然早期的实验与冷冻切片的细胞显示肌球蛋白（和肌动蛋白）的富集。α-的富集在有或没有10 mM NaN 3存在的情况下，成簇的表面分子下的辅肌动蛋白在加帽过程的早期阶段（斑块）已经很明显。细胞与不同配体的长时间孵育导致配体-受体复合物的内吞作用。α-辅肌动蛋白与内化的复合物无关，这表明它可能在胞吞过程中的某个阶段从修补或加盖的表面结构中解离。
Double fluorescence staining experiments demonstrated that intracellular .alpha.-actinin accumulates under cap and patches induced in several cells by a variety of ligands. This phenomenon was demonstrated in [mouse] lymphocytes and lymphoma cells treated with anti-H[histone]2 sera; spleen lymphocytes treated with concanavalin A or anti-immunoglobulin antibodies and VSV[vesicular stomatitis virus]-infected mouse fibroblast line MC57 treated with antiserum against viral antigens. It occurred during both rapid and slow capping processes and could be obtained by either direct or indirect ligand-induced redistribution. These observations were carried out on whole cells. For other cytoskeletal proteins such as filamin, tropomyosin and myosin, a similar accumulation under caps was not readily apparent using whole cell mounts, although earlier experiments with frozen-sectioned cells showed an enrichment of myosin (and actin). The enrichment of .alpha.-actinin under the clustered surface molecules was already apparent in early stages (patches) of the capping process, with or without 10 mM NaN3 present. Prolonged incubation of the cells with different ligands resulted in endocytosis of the ligand-receptor complex. .alpha.-Actinin was not associated with the internalized complex, suggesting that it may dissociate from the patched or capped surface structures at some stage during endocytosis.