An efficient mechanical inoculation technique for soybean dwarf virus reveals that the viral readthrough domain is inessential for the systemic infection of host plants

An efficient mechanical inoculation technique for soybean dwarf virus reveals that the viral readthrough domain is inessential for the systemic infection of host plants
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大豆矮化病毒的有效机械接种技术表明,病毒读通结构域对于宿主植物的全身感染并不重要

DOI:
10.1007/s10327-022-01057-6
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发表时间:
2022
影响因子:
1.2
通讯作者:
Hagiwara-Komoda Yuka
Hagiwara-Komoda Yuka
中科院分区:
农林科学4区
文献类型:
--
作者:
Naoki Ube;Yuhka Katsuyama;Keisuke Kariya;Shin-ichi Tebayashi;Masayuki Sue;Takuji Tohnooka;Kotomi Ueno;Shin Taketa and Atsushi Ishihara;Hagiwara-Komoda Yuka

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建立了大豆和豌豆植株机械接种大豆矮缩病毒(SbDV)的方法。将SbDV RNA转录本接种大豆和豌豆幼苗,用不锈钢细丝刺穿。2-3周后,50%以上的接种植株系统感染SbDV。用该方法对突变的SbDV接种植物的进一步分析表明,SbDV的通读结构域不是病毒在寄主植物中长距离移动所必需的。
A method for mechanical inoculation of soybean and pea plants with soybean dwarf virus (SbDV) was developed. Soybean and pea seedlings were inoculated with SbDV RNA transcript via pricks made with fine stainless steel wire. After 2–3 weeks, more than 50% of inoculated plants were systemically infected with SbDV. Further analysis using this method to inoculate plants with a mutated SbDV revealed that the readthrough domain of SbDV is not essential for viral long-distance movement in host plants.
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