Determination of penicillin V potassium in pharmaceuticals and spiked human urine by chemiluminescence
Determination of penicillin V potassium in pharmaceuticals and spiked human urine by chemiluminescence
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DOI：
10.2478/s11532-008-0092-6
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发表时间：
2009-03
影响因子：
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通讯作者：
M. Sorouraddin;M. Iranifam;A. Imani-Nabiyyi
中科院分区：
文献类型：
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作者：
M. Sorouraddin;M. Iranifam;A. Imani-Nabiyyi
建立了化学发光法测定青霉素V钾（PVK）的方法。PVK被碱性过氧化氢氧化产生CL，其被N，N-二甲基甲酰胺（DMF）和N-十六烷基-N，N，N-三甲基溴化铵（CTMAB）大大增强。采用光度法确定了最佳条件。在0.5 - 129.5 mg L-1的范围内，荧光峰高与PVK的量之间存在线性关系，检测限为0.2 mg L-1。40 mg L − 1 PVK溶液的变异系数为1.2%（n = 7）。该方法操作简单，灵敏度高，选择性好，可用于过程控制。该方法已成功用于药物和加标人尿液中PVK的测定。
A simple and selective method for penicillin V potassium (PVK) determination by chemiluminescence (CL) was developed. Oxidation of PVK by alkaline hydrogen peroxide produces CL, which is greatly enhanced by N, N-dimethyl formamide (DMF) and N-cetyl-N,N,N-trimethylammonium bromide (CTMAB). Optimum conditions were established using luminometry. There is a linear relationship between the chemiluminescent peak height and the amount of PVK within the range 0.5–129.5 mg L−1, with a detection limit of 0.2 mg L−1. The coefficient of variation was 1.2% for 40 mg L−1 PVK solution (n = 7). The method is very simple, has high sensitivity and good selectivity, and is usable for process control. It was successfully utilized for the determination of PVK in pharmaceuticals and spiked human urine.