Localization of sensory mechanisms utilized by coral planulae to detect settlement cues
Localization of sensory mechanisms utilized by coral planulae to detect settlement cues
复制标题
浮珊瑚利用感觉机制定位来检测沉降线索
DOI：
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
M. Hadfield
中科院分区：
文献类型：
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作者：
C. Tran;M. Hadfield
平坦珊瑚通过接触硬壳珊瑚藻类或海洋细菌生物膜而被诱导定居和变质。利用对不同变质线索作出反应的两种珊瑚Pocillopora Damicornis和Montipora Capitata的幼虫，研究了用于探测变质线索的感觉机制。因为珊瑚扁平线虫的极点是附着在底物上的点，所以我们预测它也是线索的检测点。为了确定线索的感觉细胞沿着身体的位置，个别幼虫被横向切割成口腔和流产部分，沿着口腔-流产轴在不同的水平上，并暴露在诱导定居的底物中。头状分枝杆菌的尾端发生变形，而口端继续游动。然而，在豆角斑潜蝇幼虫中，口端(即没有流产极点)也能够变质，这表明检测线索的细胞可能分布在身体的两侧。这些细胞并不对应于存在于全身的FMRFamide免疫反应细胞。铯离子诱导两种幼虫的流产端和口端都稳定下来，这表明口端并没有失去变质的能力，尽管缺乏感官细胞来检测自然的暗示。为了确定斑角疫霉菌幼虫的感觉细胞是否局限于身体的一侧，观察了红色荧光亲脂膜染料DiI标记的幼虫在底物上的游泳行为。发现幼虫绕着口-反轴旋转，其表面靠在底物上，不利于检测线索的特定一侧。在阐明珊瑚幼体对定居和变态至关重要的区域时，我们得出结论，珊瑚物种之间的显著差异可能是由于与不同的珊瑚大小有关的感觉结构的分布不同所致。
Coral planulae are induced to settle and metamorphose by contact with either crustose coralline algae or marine bacterial biofilms. Larvae of two coral species, Pocillopora damicornis and Montipora capitata, which respond to different metamorphic cues, were utilized to investigate the sensory mechanisms used to detect metamorphic cues. Because the aboral pole of the coral planula is the point of attachment to the substratum, we predicted that it is also the point of detection for cues. To determine where sensory cells for cues are localized along the body, individual larvae were transversely cut into oral and aboral portions at various levels along the oral–aboral axis, and exposed to settlement-inducing substrata. Aboral ends of M. capitata metamorphosed, while oral ends continued to swim. However, in larvae of P. damicornis, ¾ oral ends (i.e., lacking the aboral pole) were also able to metamorphose, indicating that the cells that detect cues may be distributed along the sides of the body. These cells do not correspond to FMRFamide-immunoreactive cells that are present throughout the body. Cesium ions induced both aboral and oral ends of larvae of both species to settle, suggesting that oral ends have not lost their capacity to metamorphose, despite lacking sensory cells to detect natural cues. To determine whether sensory cells in larvae of P. damicornis are restricted to one side of the body, swimming behavior over substrata was observed in larvae labeled with diI, a red fluorescent lipophilic membrane stain. The larvae were found to rotate around the oral–aboral axis, with their surface against the substratum, not favoring a particular side for detecting cues. While clarifying the regions of the larval body important for settlement and metamorphosis in coral planulae, we conclude that significant differences between coral species may be due to differences in the distribution of sensory structures in relation to different planular sizes.
DOI：
10.1073/pnas.0601257103
发表时间：
2006-07-25
影响因子：
11.1
作者：
Matus, David Q.;Pang, Kevin;Martindale, Mark Q.
通讯作者：
Martindale, Mark Q.