How cryoprotectants work: hydrogen-bonding in low-temperature vitrified solutions.
How cryoprotectants work: hydrogen-bonding in low-temperature vitrified solutions.
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DOI：
10.1039/d2sc03188d
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发表时间：
2022-08-31
期刊：
影响因子：
8.4
通讯作者：
中科院分区：
文献类型：
--
作者：
二甲基亚砜(DMSO)可在低温下提高细胞和组织的活力，通常用作生物材料低温储存的低温保护剂。DMSO扰乱了水的氢键网络，抑制了冰晶的生长，尽管DMSO与水的特定相互作用很难表征。在这项研究中，我们使用傅里叶变换红外光谱、分子动力学模拟和振动频率图相结合的方法来表征在30°C到−80°C范围内二甲基亚砜与水的氢键相互作用随温度的变化。模拟表明，这些被破坏的氢键网络在很大程度上仍局限于DMSO的第一水合壳，这解释了在低温保存应用中防止冰晶形成所需的高DMSO浓度。二甲基亚砜(DMSO)可在低温下提高细胞和组织的活力，通常用作生物材料低温储存的低温保护剂。
Dimethyl sulfoxide (DMSO) increases cell and tissue viability at low temperatures and is commonly used as a cryoprotectant for cryogenic storage of biological materials. DMSO disorders the water hydrogen-bond networks and inhibits ice-crystal growth, though the specific DMSO interactions with water are difficult to characterize. In this study, we use a combination of Fourier Transform infrared spectroscopy (FTIR), molecular dynamics simulations, and vibrational frequency maps to characterize the temperature-dependent hydrogen bonding interactions of DMSO with water from 30 °C to −80 °C. Specifically, broad peaks in O–D stretch vibrational spectra of DMSO and deuterated water (HDO) cosolvent systems show that the hydrogen bond networks become increasingly disrupted compared to pure water. Simulations demonstrated that these disrupted hydrogen bond networks remain largely localized to the first hydration shell of DMSO, which explains the high DMSO concentrations needed to prevent ice crystal formation in cryopreservation applications. Dimethyl sulfoxide (DMSO) increases cell and tissue viability at low temperatures and is commonly used as a cryoprotectant for cryogenic storage of biological materials.
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影响因子：
3.3
作者：
Klauda, Jeffery B.;Venable, Richard M.;Freites, J. Alfredo;O'Connor, Joseph W.;Tobias, Douglas J.;Mondragon-Ramirez, Carlos;Vorobyov, Igor;MacKerell, Alexander D., Jr.;Pastor, Richard W.
通讯作者：
Pastor, Richard W.
DOI：
10.1021/acs.jpcb.5b02936
发表时间：
2015-07-02
期刊：
The journal of physical chemistry. B
影响因子：
--
作者：
Duboué-Dijon E;Laage D
通讯作者：
Laage D
影响因子：
2.7
作者：
Fahy, Gregory M.
通讯作者：
Fahy, Gregory M.
影响因子：
16.6
作者：
Oh, Kwang-Im;Rajesh, Kavya;Baiz, Carlos R.
通讯作者：
Baiz, Carlos R.
影响因子：
4.4
作者：
Horn, HW;Swope, WC;Head-Gordon, T
通讯作者：
Head-Gordon, T