Thiopeptide biosynthesis featuring ribosomally synthesized precursor peptides and conserved posttranslational modifications.
Thiopeptide biosynthesis featuring ribosomally synthesized precursor peptides and conserved posttranslational modifications.
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DOI：
10.1016/j.chembiol.2009.01.007
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发表时间：
2009-02-27
影响因子：
--
通讯作者：
Liu W
中科院分区：
文献类型：
--
作者：
Liao R;Duan L;Lei C;Pan H;Ding Y;Zhang Q;Chen D;Shen B;Yu Y;Liu W
Thiopeptides, with potent activity against various drug-resistant pathogens, contain a characteristic macrocyclic core consisting of multiple thiazoles, dehydroamino acids, and a 6-membered nitrogen heterocycle. Their biosynthetic pathways remain elusive in spite of great efforts by in vivo feeding experiments. Here, cloning, sequencing and characterization of the thiostrepton and siomycin A gene clusters unveiled a new biosynthetic paradigm for the thiopeptide specific core formation, featuring ribosomally synthesized precursor peptides and conserved posttranslational modifications. The paradigm generality for thiopeptide biosynthesis was supported by genome mining and ultimate confirmation of the thiocillin I production in Bacillus cereus ATCC 14579, a strain that was previously unknown as a thiopeptide producer. These findings set the stage to accelerate the discovery of novel thiopeptides by prediction at the genetic level and to generate structural diversity by applying combinatorial biosynthesis methods.
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影响因子：
14.8
作者：
Donia, Mohamed S.;Hathaway, Brian J.;Schmidt, Eric W.
通讯作者：
Schmidt, Eric W.
影响因子：
3.3
作者：
EBATA, M;MIYAZAKI, K;OTSUKA, H
通讯作者：
OTSUKA, H
影响因子：
15
作者：
Nicolaou, KC;Zak, M;Nevalainen, M
通讯作者：
Nevalainen, M
DOI：
10.1073/pnas.0801338105
发表时间：
2008-04-15
影响因子：
11.1
作者：
Lee, Shaun W.;Mitchell, Douglas A.;Dixon, Jack E.
通讯作者：
Dixon, Jack E.
影响因子：
3.9
作者：
FRENZEL, T;ZHOU, P;FLOSS, HG
通讯作者：
FLOSS, HG