Metabolism of benzo[a]pyrene by fish cells in culture.
Metabolism of benzo[a]pyrene by fish cells in culture.
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DOI：
10.1080/15287398209530239
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发表时间：
1982-07
期刊：
影响因子：
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通讯作者：
S. Thornton;L. Diamond;W. Baird
中科院分区：
文献类型：
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作者：
S. Thornton;L. Diamond;W. Baird
研究了虹鳟鱼（RTG-2）、蓝鳃鱼（BF-2）和黑头鲦鱼（FHM）细胞系中苯并[a]芘（BaP）的代谢。将融合培养物暴露于3H-BaP （0.5 nmol/ml）中，在不同暴露时间后，用有机溶剂从培养基中提取代谢物，并进行高压液相色谱分析。BF-2和RTG-2细胞在24小时内将63%的BaP转化为水溶性代谢物，而FHM细胞仅转化为12%。BF-2和RTG-2细胞在48 h时代谢了90%以上的BaP，而FHM细胞在96 h后仅将67%的BaP转化为水溶性代谢物。3个细胞系的主要有机溶剂可提取代谢物为9,10-二羟基9,10-二氢苯并[a]芘和未识别的极性代谢物。在BF-2、FHM和RTG-2细胞形成的水溶性代谢物中，经β -葡萄糖醛酸酶处理后，分别有67%、42%和19%转化为乙酸乙酯可提取代谢物。所有三种细胞系形成7,8-二羟基-7,8-二氢苯并[a]芘（7,8-二醇）的葡萄糖醛酸盐；在BF-2和FHM细胞中，7,8-二醇几乎占β -葡糖醛酸酶处理释放的代谢物的一半。因此，来自三种广泛分布的淡水鱼的细胞系具有将BaP代谢为啮齿动物的一种近似致癌物的能力，并产生这种代谢物的水溶性缀合物。
Benzo[a]pyrene (BaP) metabolism was studied in cell lines derived from rainbow trout (RTG-2), bluegill fry (BF-2), and fathead minnow (FHM). Confluent cultures were exposed to 3H-BaP (0.5 nmol/ml), and, after various exposure times, metabolites were extracted from the media with an organic solvent and analyzed by high-pressure liquid chromatography. BF-2 and RTG-2 cells converted 63% of the BaP to water-soluble metabolites within 24 h, while FHM cells converted only 12%. BF-2 and RTG-2 cells metabolized more than 90% of the BaP by 48 h, while only 67% of the BaP was converted to water-soluble metabolites by FHM cells after 96 h. The major organic-solvent-extractable metabolites in all three cell lines were 9,10-dihydroxy-9,10-dihydrobenzo[a]pyrene and unidentified polar metabolites. Of the water-soluble metabolites formed by BF-2, FHM, and RTG-2 cells, 67, 42, and 19%, respectively, were converted to ethyl-acetate-extractable metabolites by treatment with beta-glucuronidase. All three cell lines formed a glucuronide of 7,8-dihydroxy-7,8-dihydrobenzo[a]pyrene (7,8-diol); in BF-2 and FHM cells, the 7,8-diol represented almost half of the metabolites released by beta-glucuronidase treatment. Thus, cell lines derived from three widely distributed species of freshwater fish have the capacity to metabolize BaP to a form that is a proximate carcinogen in rodents and to produce a water-soluble conjugate of this metabolite.