Reliability of the dye penetration studies
Reliability of the dye penetration studies
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DOI：
10.1097/00004770-200309000-00012
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发表时间：
2003-09-01
影响因子：
4.2
通讯作者：
Pashley, D
中科院分区：
文献类型：
--
作者：
Camps, J;Pashley, D
本研究的目的是比较经典的染料渗透法，染料萃取法，与液体过滤法作为对照。使用ProFile器械准备40颗牙齿，并根据用于侧向加压的器械分为4组（每组n = 10）：髓腔封闭器、Sealapex、AH Plus和Ketac-Endo。用所有三种方法在相同的牙齿上评价根尖封闭，依次为：液体过滤法、2%亚甲基蓝染料渗透法和一种新方法，其中在阅读溶液的吸光度之前将牙根溶解在65%硝酸中以提取亚甲基蓝。经典染料渗透在封闭剂之间没有显示出任何差异，并且与其他两种技术没有相关性。液体过滤（p <0.01）和染料浸提（p <0.01）显示Sealapex显示出最高的根尖渗漏。用这两种方法获得的结果之间的相关性是显著的（p = 0.001和r = 0.7）。这项研究表明，传统的染料渗透研究的局限性，染料提取，即溶解，方法给出了相同的结果，流体过滤，但节省了大量的实验室时间。
The purpose of this study was to compare the classical dye-penetration method to a dye-extraction method, with a fluid-filtration method as control. Forty teeth were prepared with a ProFile device and divided into four groups (n = 10 per group) according to the sealer used for the lateral condensation: Pulp Canal Sealer, Sealapex, AH Plus, and Ketac-Endo. The apical seal was evaluated on the same teeth with all three methods, successively: a fluid-filtration method, a dye-penetration method with 2% methylene blue, and a new method where the roots were dissolved in 65% nitric acid to extract the methylene blue before reading the absorbance of the solution. The classical dye penetration did not show any difference among the sealers and showed no correlation with the two other techniques. The fluid filtration (p < 0.01) and the dye extraction (p < 0.01) showed that Sealapex displayed the highest apical leakage. The correlation between the results obtained with these two methods was significant (p = 0.001 and r = 0.7). This study showed the limitation of the classical dye-penetration studies and that the dye-extraction, i.e. dissolution, method gave the same results as fluid filtration but saved much laboratory time.