Multi-colored homologs of the green fluorescent protein from hydromedusa Obelia sp.
Multi-colored homologs of the green fluorescent protein from hydromedusa Obelia sp.
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DOI：
10.1039/c1pp05068k
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发表时间：
2011-01-01
影响因子：
3.1
通讯作者：
Matz, Mikhail V.
中科院分区：
文献类型：
--
作者：
Aglyamova, Galina V.;Hunt, Marguerite E.;Matz, Mikhail V.
绿色荧光蛋白（GFP）是在水母类中发现后不久被推测存在于Obelia（刺胞动物门，水螅动物门，钟形虫科）的生物发光系统中的。尽管水母GFP作为遗传编码荧光标记取得了巨大的成功，但Obelia GFP迄今为止一直在挫败从水螅生命周期阶段克隆它的尝试。在这里，我们报告的三个GFP样荧光蛋白（FP）从Obelia水母，代表青色，绿色，黄色光谱类型的克隆。这种颜色的多样性从来没有被检测到以外的类珊瑚虫，这表明一个更普遍的功能，多色荧光刺胞动物比以前假设的。新FP的一个不寻常的特性是形成具有明确尺寸和分子量的大的可溶性复合物，对应于多达128个单独的多肽。这与早期的观察结果一致，即与水母不同，Obelia中的发光位于亚细胞颗粒内，这促使进一步探究新FP的自组装特性及其与发光蛋白的相互作用。Obelia FPs的发现填补了刺胞动物生物发光知识的40年空白，并为进一步研究其分子机制的细节提供了实验材料。
The presence of green fluorescent protein (GFP) within the bioluminescent system of Obelia (Cnidaria, Hydrozoa, Campanulariidae) was inferred shortly after the discovery of GFP in Aequorea. Despite the enormous success of Aequorea GFP as a genetically encoded fluorescent label, Obelia GFP thus far has been defeating attempts to clone it from the hydroid life cycle stage. Here, we report cloning of three GFP-like fluorescent proteins (FPs) from Obelia medusa, representing cyan, green, and yellow spectral types. Such color diversity has never been detected outside class Anthozoa, suggesting a more general function for multi-colored fluorescence in cnidarians than has been previously hypothesized. An unusual property of the new FPs is the formation of large soluble complexes of well-defined sizes and molecular weights, corresponding to up to 128 individual polypeptides. This aligns well with the earlier observation that luminescence in Obelia, unlike in Aequorea, is localized within subcellular granules, which prompts further inquiry into the self-assembly properties of the new FPs and their interactions with the photoprotein. The discovery of Obelia FPs fills the four-decade-old gap in the knowledge of cnidarian bioluminescence and provides experimental material to further investigate the details of its molecular mechanism.