Sequences and studies of bacteriophage T4 rII mutants.
Sequences and studies of bacteriophage T4 rII mutants.
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噬菌体 T4 rII 突变体的序列和研究。
DOI：
10.1016/0022-2836(87)90176-8
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发表时间：
1987
影响因子：
5.6
通讯作者：
Gold,L
中科院分区：
文献类型：
--
作者：
Shinedling,S;Singer,BS;Gayle,M;Pribnow,D;Jarvis,E;Edgar,B;Gold,L
我们已经对T4噬菌体RIIA和RIIB基因的80多个突变体进行了测序。这些突变包括：我们推测其来源的缺失；影响RIIb启动子的突变；RII−表型的各种伪回复突变，包括导致终止后重新启动翻译的突变；影响RIIb翻译调控的突变；有毒的微小斑块突变体FC237和FC238及其解毒剂；以及更多来自剑桥收藏的经典移码突变。这些突变体已经通过以单链DNA为模板的大肠杆菌DNA聚合酶或以mRNA或DNA为模板的禽类逆转录病毒逆转录酶进行了双脱氧链终止测序。我们列出了突变体的序列变化和相关的历史和表型数据。我们讨论了促进翻译重新启动的突变，并讨论了一个可以解释许多突变的产生的模型。
We have sequenced more than 80 mutants of the bacteriophage T4 rIIA and rIIB genes. These include deletions about whose origin we have speculated, mutations affecting the rIIB promoters, various pseudo-revertants of the rII−phenotype, including mutations that bring about the reinitiation of translation following termination, mutations that affect regulation of rIIB translation byregA, the toxic minute plaquing mutants FC237 and FC238 and their detoxifiers, and many more of the classic frameshifts from the Cambridge collection. These mutants have been sequenced using dideoxy-mediated chain termination by eitherEscherichia coliDNA polymerase using single-stranded DNA as a template or by avian retroviral reverse transcriptase using mRNA or DNA as the template molecule. We list the sequence changes of the mutants with pertinent historic and phenotypic data. The mutants that facilitate translation reinitiation are discussed, and we discuss a model that could account for the generation of many of the mutations.