Activity-Induced Long-Term Potentiation of Excitatory Synapses in Developing Zebrafish Retina In Vivo
Activity-Induced Long-Term Potentiation of Excitatory Synapses in Developing Zebrafish Retina In Vivo
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DOI：
10.1016/j.neuron.2012.05.031
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发表时间：
2012-08
期刊：
影响因子：
16.2
通讯作者：
Hong-ping Wei;Yuan Yao;Rong-wei Zhang;Xiao-Feng Zhao;Jiu-Lin Du
中科院分区：
文献类型：
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作者：
Hong-ping Wei;Yuan Yao;Rong-wei Zhang;Xiao-Feng Zhao;Jiu-Lin Du
神经活动诱导的突触传递长时程增强（LTP）被认为是神经回路经验依赖性发育完善的细胞机制之一。虽然视觉体验和神经活动对于视网膜回路的完善至关重要，但LTP是否以及如何在视网膜中发生仍然未知。使用在体穿孔全细胞记录和双光子钙成像，我们发现，重复的电刺激和视觉刺激可以诱导LTP在兴奋性突触形成的双极细胞视网膜神经节细胞在幼体，但不是少年斑马鱼。LTP诱导需要激活突触后N-甲基-D-天冬氨酸受体，其表达涉及花生四烯酸依赖性突触前钙动力学和神经递质释放的变化。在生理上，电刺激和视觉刺激诱导的LTP都能增强视网膜神经节细胞的视觉反应。因此，LTP存在于发育中的视网膜中，具有突触前位点，并且可能有助于视网膜回路的视觉体验依赖性细化。
Neural activity-induced long-term potentiation (LTP) of synaptic transmission is believed to be one of the cellular mechanisms underlying experience-dependent developmental refinement of neural circuits. Although it is well established that visual experience and neural activity are critical for the refinement of retinal circuits, whether and how LTP occurs in the retina remain unknown. Using in vivo perforated whole-cell recording and two-photon calcium imaging, we find that both repeated electrical and visual stimulations can induce LTP at excitatory synapses formed by bipolar cells on retinal ganglion cells in larval but not juvenile zebrafish. LTP induction requires the activation of postsynapticN-methyl-D-aspartate receptors, and its expression involves arachidonic acid-dependent presynaptic changes in calcium dynamics and neurotransmitter release. Physiologically, both electrical and visual stimulation-induced LTP can enhance visual responses of retinal ganglion cells. Thus, LTP exists in developing retinae with a presynaptic locus and may serve for visual experience-dependent refinement of retinal circuits.