Stability and inactivation of HTLV-III/LAV under clinical and laboratory environments.
Stability and inactivation of HTLV-III/LAV under clinical and laboratory environments.
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HTLV-III/LAV 在临床和实验室环境下的稳定性和灭活。
DOI：
10.1001/jama.1986.03370140085029
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
P. Markham
中科院分区：
文献类型：
--
作者：
L. Resnick;K. Veren;S. Salahuddin;S. Tondreau;P. Markham
研究了人 T 细胞嗜淋巴细胞病毒 III 型/淋巴结病相关病毒 (HTLV-III/LAV) 在临床或实验室环境条件下的稳定性以及常用化学消毒剂对其的灭活作用。在我们使用高浓缩病毒制剂的实验条件下，初始感染滴度约为每毫升 7 log10 组织培养感染剂量 (TCID50) 的病毒可以从室温（23 至 27 摄氏度）或 36 至 37 摄氏度的水环境中回收一周以上。当保持在 54 至 56 摄氏度时，病毒回收率以每 20 分钟约 1 log10 TCID50 的速度减少。在室温下保存，HTLV-III/LAV 的传染性可保留三天以上，每 9 小时减少约 1 log10TCID50。使用 0.5% 次氯酸钠、70% 酒精或 0.5% nonidet-P40 时，病毒感染性在一分钟内无法检测到，并且使用 0.08% 氯化季铵或 1:1 丙酮-酒精混合物时，病毒感染性在 10 分钟内降低超过 7 log10TCID50。这些结果有助于为实验室或临床环境中防止 HTLV-III/LAV 意外传播提供合理依据。
The stability of human T-cell lymphotropic virus type III/lymphadenopathy-associated virus (HTLV-III/LAV) under environmental conditions encountered in a clinical or laboratory setting and its inactivation by commonly used chemical disinfectants were investigated. Under our experimental conditions utilizing a highly concentrated viral preparation, virus with an initial infectious titer of approximately 7 log10 tissue culture infectious dose (TCID50) per milliliter can be recovered for more than a week from an aqueous environment held at room temperature (23 to 27 degrees C) or at 36 to 37 degrees C. Virus recovery is reduced at a rate of approximately 1 log10TCID50 per 20 minutes when held at 54 to 56 degrees C. Dried and held at room temperature, HTLV-III/LAV retains infectivity for more than three days with a reduction of approximately 1 log10TCID50 per nine hours. Viral infectivity is undetectable and reduced more than 7 log10TCID50 within one minute with 0.5% sodium hypochlorite, 70% alcohol, or 0.5% nonidet-P40, and within ten minutes with 0.08% quaternary ammonium chloride or with a 1:1 mixture of acetone-alcohol. These results help provide a rational basis to prevent the accidental spread of HTLV-III/LAV in the laboratory or clinical setting.