Origin of class 1 and 2 integrons and gene cassettes in a population-based sample of uropathogenic Escherichia coli.
Origin of class 1 and 2 integrons and gene cassettes in a population-based sample of uropathogenic Escherichia coli.
复制标题
尿路致病性大肠杆菌群体样本中 1 类和 2 类整合子和基因盒的起源。
DOI：
10.1128/jcm.44.4.1347-1351.2006
复制
发表时间：
2006
影响因子：
9.4
通讯作者：
Riley,LeeW
中科院分区：
文献类型：
--
作者：
Solberg,OwenD;Ajiboye,RemiM;Riley,LeeW
The prevalence of urinary tract infections (UTI) caused by trimethoprim-sulfamethoxazole (TMP-SMX)-resistantEscherichia coliis increasing and varies geographically in the United States. Recent community-based UTI studies have demonstrated geographic clustering of anEscherichia coliclonal group, suggesting occurrence of a community outbreak of UTI. A large proportion of this clonal group (designated CgA) isolated from women in a California college community was found to be resistant to TMP-SMX. We wished to determine if the acquisition of TMP-SMX resistance by CgA occurred before or after the CgA strains were introduced into this community. Between October 1999 and January 2000 and between October 2000 and January 2001, 482E. coliisolates were consecutively collected from the urine samples of women with UTI at a student health clinic and analyzed for determinants of TMP-SMX resistance. In particular, the distribution of integrons harboring resistance cassettes for TMP-SMX (dfr) was examined. Among 95 TMP-SMX-resistant isolates, 68 and 27 isolates carried class 1 and class 2 integrons, respectively. A class 1 integron was found in 25 (93%) of 27 TMP-SMX-resistant CgA isolates but in only 43 (63%) of 68 TMP-SMX-resistant non-CgA isolates (P< 0.001) and in none of 44 TMP-SMX-susceptibleE. coliisolates (P< 0.0001). CgA strains carried only a single arrangement of class 1 gene cassettes (dfrA17-aadA5), while the non-clonal group strains carried nine different cassette arrangements. These results support the idea that CgA strains acquired their resistance at a common site prior to their spread to the college community.
