Circulating miRNA-1290 as a potential biomarker for response to chemoradiotherapy and prognosis of patients with advanced oral squamous cell carcinoma: A single-center retrospective study.
Circulating miRNA-1290 as a potential biomarker for response to chemoradiotherapy and prognosis of patients with advanced oral squamous cell carcinoma: A single-center retrospective study.
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DOI：
10.1177/1010428319826853
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发表时间：
2019-03-01
期刊：
影响因子：
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通讯作者：
Nakayama, Hideki
中科院分区：
文献类型：
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作者：
Nakashima, Hikaru;Yoshida, Ryoji;Nakayama, Hideki
MicroRNA是一类小的内源性非编码18至24个核苷酸长的RNA，可以调节与癌症进展相关的多种过程。然而，其在口腔鳞状细胞癌患者中的临床价值尚未得到充分探讨。因此，本研究的目的是探讨口腔鳞癌患者循环microRNA的临床意义。采用实时定量聚合酶链反应检测健康志愿者和口腔鳞状细胞癌患者中循环miR-1246和miR-1290的表达水平。还通过定量实时聚合酶链反应检测了放射抗性口腔鳞状细胞癌细胞系（SAS-R）和亲本细胞系（SAS）以及从这些细胞系获得的条件培养基中两种microRNA的表达水平。此外，我们还对55例局部晚期口腔鳞状细胞癌患者的循环microRNA状态与各种临床病理特征之间的相关性进行了研究，这些患者在接受了5-氟尿嘧啶为基础的放化疗后接受了手术。口腔鳞癌患者血浆中miR-1290的表达水平显著低于健康志愿者（p
MicroRNAs are a class of small, endogenous, noncoding 18- to 24-nucleotide-long RNAs that can regulate multiple processes related to cancer progression. However, their clinical value in patients with oral squamous cell carcinoma has not yet been fully explored. Therefore, the aim of this study was to investigate the clinical significance of circulating microRNAs in oral squamous cell carcinoma patients. The expression levels of circulating miR-1246 and miR-1290 in healthy volunteers and oral squamous cell carcinoma patients were examined by quantitative real-time polymerase chain reaction. The expression levels of both microRNAs in the radioresistant oral squamous cell carcinoma cell line (SAS-R) and the parent cell line (SAS) and in the conditioned medium obtained from these cell lines were also examined by quantitative real-time polymerase chain reaction. In addition, the correlations between circulating microRNA status and various clinicopathological features in 55 oral squamous cell carcinoma patients with locally advanced oral squamous cell carcinoma who underwent surgery following 5-fluorouracil-based chemoradiotherapy were examined. The expression level of miR-1290 was significantly lower in the plasma of oral squamous cell carcinoma patients than in that of healthy volunteers (p