Virus-like particles displaying H5, H7, H9 hemagglutinins and N1 neuraminidase elicit protective immunity to heterologous avian influenza viruses in chickens.
Virus-like particles displaying H5, H7, H9 hemagglutinins and N1 neuraminidase elicit protective immunity to heterologous avian influenza viruses in chickens.
复制标题
表现出H5，H7，H9血凝素和N1神经氨酸酶的病毒样颗粒会引起对鸡的异源禽流感病毒的保护性免疫。
DOI：
10.1016/j.virol.2016.12.001
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发表时间：
2017-01-15
期刊：
影响因子：
3.7
通讯作者：
Kapczynski DR
中科院分区：
文献类型：
--
作者：
Pushko P;Tretyakova I;Hidajat R;Zsak A;Chrzastek K;Tumpey TM;Kapczynski DR
在野生鸟类中传播的禽流感（AI）病毒对公众健康构成严重威胁。需要针对人工智能亚型的人类和兽用疫苗。在这里，我们制备了三亚型 VLP，其共定位源自 H5N1、H7N3 和 H9N2 病毒的 H5、H7 和 H9 抗原。 VLP 还含有 N1 型流感神经氨酸酶和逆转录病毒 gag 蛋白。使用杆状病毒表达制备H5/H7/H9/N1/gag VLP。测定生化、功能和抗原特征，包括血凝和神经氨酸酶活性。在鸡 AI 攻击模型中进一步评估了 VLP 对异源 AI 病毒（包括 H5N2、H7N3 和 H9N2 亚型）的安全性、免疫原性和保护功效。所有接种疫苗的鸟类都在 H5N2 和 H7N3 高致病性 AI (HPAI) 病毒的挑战中幸存下来，而所有对照组均死亡。用低致病性 AI (LPAI) H9N2 病毒攻击后也可检测到免疫反应，这表明 H5/H7/H9/N1/gag VLP 代表了开发广泛保护性 AI 疫苗的一种有前途的方法。
Avian influenza (AI) viruses circulating in wild birds pose a serious threat to public health. Human and veterinary vaccines against AI subtypes are needed. Here we prepared triple-subtype VLPs that co-localized H5, H7 and H9 antigens derived from H5N1, H7N3 and H9N2 viruses. VLPs also contained influenza N1 neuraminidase and retroviral gag protein. The H5/H7/H9/N1/gag VLPs were prepared using baculovirus expression. Biochemical, functional and antigenic characteristics were determined including hemagglutination and neuraminidase enzyme activities. VLPs were further evaluated in a chicken AI challenge model for safety, immunogenicity and protective efficacy against heterologous AI viruses including H5N2, H7N3 and H9N2 subtypes. All vaccinated birds survived challenges with H5N2 and H7N3 highly pathogenic AI (HPAI) viruses, while all controls died. Immune response was also detectable after challenge with low pathogenicity AI (LPAI) H9N2 virus suggesting that H5/H7/H9/N1/gag VLPs represent a promising approach for the development of broadly protective AI vaccine.