Three-Dimensional Micro-MRI Analysis of Cerebral Artery Development in Mouse Embryos
Three-Dimensional Micro-MRI Analysis of Cerebral Artery Development in Mouse Embryos
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DOI：
10.1002/mrm.22113
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发表时间：
2009-12-01
影响因子：
3.3
通讯作者：
Turnbull, Daniel H.
中科院分区：
文献类型：
--
作者：
Berrios-Otero, Cesar A.;Wadghiri, Youssef Zaim;Turnbull, Daniel H.
维管系统的发育涉及一个复杂的三维分支过程，这对正常的胚胎发生至关重要。在大脑中，动脉系统似乎以定型的方式发育，但没有详细的定量分析小鼠胚胎脑动脉的描述。在这项研究中，钆基造影剂灌注方法的发展，选择性地增强脑动脉在固定的小鼠胚胎。三维磁共振显微成像（micro-MRI）数据同时从多个胚胎分期之间的10和17天的妊娠，和各种图像分析方法被用来提取和分析脑动脉模式。结果表明，小鼠大脑中的主要动脉分支在个体之间非常相似，早期建立的模式和生长通过节段的延伸发生，同时保持基础的血管几何形状。为了研究该方法用于突变小鼠表型分析的实用性，从Gli 2（-/-）突变胚胎及其野生型同窝仔中获得对比增强的micro-MRI数据，显示Gli 2（-/-）胚胎中的几种先前未报道的血管表型，包括基底动脉的完全缺失。这些结果表明，对比度增强的micro-MRI提供了一个强大的工具，用于分析各种基因工程小鼠的血管表型。Magn Reson Med 62：1431-1439，2009. (C)2009年Wiley-Liss，Inc.
Vascular system development involves a complex, three-dimensional branching process that is critical for normal embryogenesis. In the brain, the arterial systems appear to develop in a stereotyped fashion, but no detailed quantitative analyses of the mouse embryonic cerebral arteries have been described. In this study, a gadolinium-based contrast perfusion method was developed to selectively enhance the cerebral arteries in fixed mouse embryos. Three-dimensional magnetic resonance micro-imaging (micro-MRI) data were acquired simultaneously from multiple embryos staged between 10 and 17 days of gestation, and a variety of image analysis methods was used to extract and analyze the cerebral arterial patterns. The results show that the primary arterial branches in the mouse brain are very similar between individuals, with the patterns established early and growth occurring by extension of the segments, while maintaining the underlying vascular geometry. To investigate the utility of this method for mutant mouse phenotype analysis, contrast-enhanced micro-MRI data were acquired from Gli2(-/-) mutant embryos and their wild-type littermates, showing several previously unreported vascular phenotypes in Gli2(-/-) embryos, including the complete absence of the basilar artery. These results demonstrate that contrast-enhanced micro-MRI provides a powerful tool for analyzing vascular phenotypes in a variety of genetically engineered mice. Magn Reson Med 62: 1431-1439, 2009. (C) 2009 Wiley-Liss, Inc.