A rapid analytical method for hair analysis using ambient pressure ion mobility mass spectrometry with electrospray ionization (ESI-IMMS)
A rapid analytical method for hair analysis using ambient pressure ion mobility mass spectrometry with electrospray ionization (ESI-IMMS)
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DOI：
10.1007/s12127-008-0006-5
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发表时间：
2008-12-01
影响因子：
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通讯作者：
Hill, Herbert H., Jr.
中科院分区：
文献类型：
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作者：
Dwivedi, Prabha;Hill, Herbert H., Jr.
毛发分析通常用于评估药物滥用史、暴露于环境和工业污染物、重金属、妊娠期药物暴露和各种其他筛查目的。本文报道了电喷雾离子源大气压离子迁移谱质谱（IMMS）在头发分析中的应用。该研究表明，离子迁移谱（IMS）作为质谱（MS）分析之前的预分离技术，可检测和测定头发中纳米摩尔浓度水平的感兴趣化合物。从毛发中提取分析物后，ESI-IMMS分析方法不需要衍生化或样品处理，而这通常需要其他分离方法，如气相色谱法。IMS相对于色谱分离的一个优点是分辨能力与GC中的分辨能力相似，并且比液相色谱可能的分辨能力大得多。此外，分离速度比气相色谱法和液相色谱法都快。在当地一家发廊的顾客匿名捐赠的9份头发样本中，有4份检测出咖啡因阳性，4份检测出咖啡因阳性的样本中有2份也检测出尼古丁阳性。根据对甲基苯丙胺的质量分析，其中一份头发样本得到了阳性反应。使用迁移率数据的进一步研究表明，该反应是假阳性，可能是使用发胶所致。本文报道了IMMS作为快速和常规筛查头发样品，化妆品和药品的分析技术的潜力。
Analysis of hair is often applied to assess drug abuse history, exposure to environmental and industrial pollutants, heavy metals, gestational drug exposure and various other screening purposes. This manuscript reports the application of ambient pressure ion mobility spectrometry mass spectrometry (IMMS) with electrospray ionization (ESI) source as a rapid analytical tool for hair analysis. The study demonstrated that ion mobility spectrometry (IMS) as a pre-separation technique prior to analysis by mass spectrometry (MS) provides detection and determination of compounds of interest present in hair at nano-molar concentration level. After extraction of analytes from hair, the ESI-IMMS method of analysis does not require the derivatization or sample treatment that is often required for other separation methods such as gas chromatography. One advantage of IMS over chromatography separation is that resolving powers are similar to those in GC and much greater than those possible by liquid chromatography. In addition, separation speed is faster than both gas and liquid chromatographic methods. Four of the nine hair samples anonymously donated by customers at a local hair salon tested positive for caffeine and two of the four samples that tested positive for caffeine also tested positive for nicotine. A positive response based on mass analysis for methamphetamine was obtained for one of the hair samples. Further investigation using the mobility data demonstrated that the response was a false positive and that it may have occurred from the use of a hair gel. This article reports the potential of IMMS as an analytical technique for rapid and routine screening of hair samples, cosmetics, and pharmaceuticals.