Getting the label in: practical research strategies for tracing dietary fat.
Getting the label in: practical research strategies for tracing dietary fat.
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获取标签：追踪膳食脂肪的实用研究策略。
DOI：
10.1038/ijosup.2012.22
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Parks,EJ
中科院分区：
文献类型：
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作者：
Lambert,JE;Parks,EJ
过去 40 年来，人们对餐后发生的事件可直接影响代谢风险的观察越来越感兴趣。因此，研究人员进行餐后研究的愿望也随之增加。与膳食数量和成分选择相关的研究设计决策比显而易见的要困难得多，现在文献中有充足的证据表明，测试当天的饮食会显着影响餐后代谢，因此会影响对结果的解释。此外，测试日之前发生的事件（食物摄入和活动）也会对观察到的餐后反应产生影响。本综述的目的是介绍对餐后代谢研究至关重要的研究设计方面。这些细节包括受试者准备、膳食量、形式和成分，以及用于测量代谢物的采样方案。提供了关键因素和实际例子，以尽量减少非研究变量对受试者变异性的影响。最后，讨论了与使用稳定同位素示踪剂测量膳食脂肪代谢相关的方面，包括示踪剂形式、剂量和在食物中的递送的选择。鉴于联邦事件对慢性疾病风险有重大影响，改进研究食物能量吸收和处理的方法将支持制定旨在预防和治疗与营养过度消耗相关的疾病的策略。
The observation that events occurring after consumption of a meal can directly affect metabolic risk has been gaining interest over the past 40 years. As a result, the desire for investigators to conduct postprandial studies has also increased. Study design decisions pertaining to the choice of meal quantity and composition are more difficult than may be readily apparent, and there is now ample evidence available in the literature to suggest that what is fed on the test day significantly affects postprandial metabolism and can therefore influence interpretation of results. In addition, events occurring before the testing day (food intake and activities) can also have an impact on the observed postprandial response. The goal of this review is to present aspects of study design critical to the investigation of postprandial metabolism. These details include subject preparation, meal quantity, form and composition, as well as sampling protocols for measuring metabolites. Key factors and practical examples are provided to minimize the impact of nonresearch variables on subject variability. Finally, aspects related to using stable isotope tracers to measure metabolism of meal fat are discussed, including choice of tracer form, dose and delivery in food. Given that fed-state events contribute significantly to chronic disease risk, improved methods to study the absorption and disposal of food energy will support the development of strategies designed to prevent and treat diseases associated with overconsumption of nutrients.