Glycan Stimulation Enables Purification of Prostate Cancer Circulating Tumor Cells on PEDOT NanoVelcro Chips for RNA Biomarker Detection.
Glycan Stimulation Enables Purification of Prostate Cancer Circulating Tumor Cells on PEDOT NanoVelcro Chips for RNA Biomarker Detection.
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DOI：
10.1002/adhm.201700701
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发表时间：
2018-03
影响因子：
10
通讯作者：
Yu HH
中科院分区：
文献类型：
--
作者：
Shen MY;Chen JF;Luo CH;Lee S;Li CH;Yang YL;Tsai YH;Ho BC;Bao LR;Lee TJ;Jan YJ;Zhu YZ;Cheng S;Feng FY;Chen P;Hou S;Agopian V;Hsiao YS;Tseng HR;Posadas EM;Yu HH
构建了聚(3，4-乙二氧基噻吩基)S(PEDOT)纳米材料平台，用于纯化前列腺癌(PCa)患者血液中循环肿瘤细胞(CTCs)。这个新的平台，苯基硼酸(PBA)接枝的PEDOT NanoVelcro，将大大提高CTC捕获效率的3D PEDOT纳米基质与聚(EDOT-PBA-co-EDOT-EG3)界面层结合在一起，不仅在抗体结合时为CTC捕获提供了高特异性，而且使山梨醇能够竞争性结合，温和地释放捕获的细胞。通过这种PEDOT NanoVelcro芯片纯化的CTC提供了保存良好的RNA转录本，用于分析几个PCA特异性RNA生物标志物的表达水平，这可能为临床提供对疾病的洞察。
A glycan-stimulated and poly(3,4-ethylene-dioxythiophene)s (PEDOT)-based nanomaterial platform is fabricated to purify circulating tumor cells (CTCs) from blood samples of prostate cancer (PCa) patients. This new platform, phenylboronic acid (PBA)-grafted PEDOT NanoVelcro, combines the 3D PEDOT nanosubstrate, which greatly enhances CTC capturing efficiency, with a poly(EDOT-PBA-co-EDOT-EG3) interfacial layer, which not only provides high specificity for CTC capture upon antibody conjugation but also enables competitive binding of sorbitol to gently release the captured cells. CTCs purified by this PEDOT NanoVelcro chip provide well-preserved RNA transcripts for the analysis of the expression level of several PCa-specific RNA biomarkers, which may provide clinical insights into the disease.
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影响因子：
13.3
作者：
Hsiao, Yu-Sheng;Luo, Shyh-Chyang;Hou, Shuang;Zhu, Bo;Sekine, Jun;Kuo, Chiung-Wen;Chueh, Di-Yen;Yu, Hsiao-hua;Tseng, Hsian-Rong;Chen, Peilin
通讯作者：
Chen, Peilin
影响因子：
158.5
作者：
Cristofanilli, M;Budd, GT;Hayes, DF
通讯作者：
Hayes, DF
影响因子：
11.5
作者：
Danila, Daniel C.;Heller, Glenn;Scher, Howard I.
通讯作者：
Scher, Howard I.
影响因子：
12.3
作者：
Heitzer E;Auer M;Ulz P;Geigl JB;Speicher MR
通讯作者：
Speicher MR
DOI：
10.1056/nejmoa1315815
发表时间：
2014-09-11
期刊：
The New England journal of medicine
影响因子：
--
作者：
Antonarakis ES;Lu C;Wang H;Luber B;Nakazawa M;Roeser JC;Chen Y;Mohammad TA;Chen Y;Fedor HL;Lotan TL;Zheng Q;De Marzo AM;Isaacs JT;Isaacs WB;Nadal R;Paller CJ;Denmeade SR;Carducci MA;Eisenberger MA;Luo J
通讯作者：
Luo J