Effect of hypothermic perfusion on corneal endothelial morphology.
Effect of hypothermic perfusion on corneal endothelial morphology.
复制标题
低温灌注对角膜内皮形态的影响。
DOI：
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发表时间：
1983
影响因子：
4.1
通讯作者：
Donald R. May
中科院分区：
文献类型：
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作者：
M. Mannis;Robert Miller;Edward C. Carlson;Dave M. Hinds;Donald R. May
适度的体内低温灌注对角膜内皮完整性的影响进行了研究，在猫。11只猫进行了30分钟的体内前房灌注与常温（23摄氏度）或低温（5摄氏度）灌注液。然后对角膜进行临床（生物显微镜检查）、功能（活体染色）和形态学（扫描电子显微镜检查）评估，以了解低温灌注引起的变化。所有3种评价模式均表明在2种实验灌注条件下角膜内皮完整性无差异。在临床和扫描电镜水平上，低温灌注对角膜内皮细胞没有任何影响。局部低温在涉及长时间眼内灌注的手术中具有理论和潜在的实用性。
The effect of moderate in-vivo hypothermic perfusion on corneal endothelial integrity was studied in the cat. Eleven cats underwent in-vivo anterior chamber perfusion for 30 minutes with either normothermic (23 degrees C) or hypothermic (5 degrees C) perfusate. Corneas were then evaluated clinically (biomicroscopy), functionally (vital staining), and morphologically (scanning electron microscopy) for changes attributable to hypothermic perfusion. All 3 modes of evaluation suggested no difference in corneal endothelial integrity under the 2 experimental perfusion conditions. At the clinical and scanning electron microscope levels hypothermic perfusion does not show any effects on the corneal endothelium. Regional hypothermia is of theoretical and potential utility in procedures involving prolonged intraocular perfusion.