Cell surface sialoprotein alterations in metastatic murine colon cancer cell lines selected in an animal model for colon cancer metastasis.
Cell surface sialoprotein alterations in metastatic murine colon cancer cell lines selected in an animal model for colon cancer metastasis.
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发表时间：
1990-02
期刊：
影响因子：
11.2
通讯作者：
R. Bresalier;R. Rockwell;R. Dahiya;Q. Duh;Y. Kim
中科院分区：
文献类型：
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作者：
R. Bresalier;R. Rockwell;R. Dahiya;Q. Duh;Y. Kim
细胞表面蛋白和糖蛋白的改变可能在决定肿瘤细胞的转移行为中起关键作用。在结肠癌转移的动物模型中选择的一系列相关小鼠结肠癌细胞的细胞表面蛋白（R. S. Bresalier等人，癌症研究所，47：1398-1406，1987），因此通过多种生物化学方法进行比较。乳过氧化物酶催化的碘化的细胞表面蛋白，然后由十二烷基硫酸钠-聚丙烯酰胺凝胶电泳表现出定量和定性的差异，在细胞表面蛋白质的父母细胞系51 B（低转移潜能）和其转移衍生物51 B LiM 5和51 B LiM 6。含唾液酸的蛋白质的标记表明，在这些蛋白质中的至少四种（Mr 170，000、120，000、95，000和55，000）的情况下，这表示来自转移性细胞系的唾液酸糖蛋白的放射性标记增加。亲和层析的溶解125 I标记的细胞膜蛋白揭示了2- 3倍增加麦胚凝集素和黑接骨木凝集素结合与转移线，相比，转移性差的父母。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳从这些列洗脱的材料表明增强蛋白质从转移细胞对应的分子量，以前确定的主要唾液酸糖蛋白。神经氨酸酶可释放的膜相关的唾液酸和唾液酸转移酶的活动是2- 3倍高的转移性细胞系相比，父母线。通过预先去除细胞表面唾液酸，将各种品系脾内注射到同基因小鼠中后的肝脏定植显著减少。盲肠注射亲本51 B后形成的原发性和转移性肿瘤的免疫组织化学染色表明麦胚凝集素结合肿瘤细胞的选择性转移。这些结果进一步支持了细胞膜唾液酸化在决定癌细胞的转移潜能中是重要的概念。
Alterations in cell surface proteins and glycoproteins may play a key role in determining the metastatic behavior of tumor cells. The cell surface proteins of a series of related murine colon cancer cells selected in an animal model for colon cancer metastasis (R. S. Bresalier et al., Cancer Res., 47: 1398-1406, 1987) were therefore compared by a variety of biochemical methods. Lactoperoxidase-catalyzed iodination of cell surface proteins followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis demonstrated quantitative and qualitative differences in the cell surface protein profiles of parental cell line 51B (low metastatic potential) and its metastatic derivatives 51B LiM 5 and 51B LiM 6. Labeling of sialic acid-containing proteins suggested that, in the case of at least four of these proteins (Mr 170,000, 120,000, 95,000, and 55,000), this represented an increase in radioactive labeling of sialoglycoproteins from the metastatic lines. Affinity chromatography of solubilized 125I-labeled cell membrane proteins revealed a 2- to 3-fold increase in wheat germ agglutinin and Sambucus nigra lectin binding associated with the metastatic lines, compared to the poorly metastatic parent. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of material eluted from these columns demonstrated enhancement of proteins from the metastatic cells corresponding in molecular weight to the previously identified major sialoglycoproteins. Neuraminidase-releasable membrane-associated sialic acid and sialyltransferase activities were 2- to 3-fold higher in the metastatic cell lines compared to the parental line. Liver colonization after intrasplenic injection of the various lines into syngeneic mice was dramatically reduced by prior removal of cell surface sialic acid. Immunohistochemical staining of primary and metastatic tumors formed after cecal injection of parental 51B suggested selective metastasis by wheat germ agglutinin-binding tumor cells. These results further support the concept that cell membrane sialylation is important in determining the metastatic potential of cancer cells.