Activation of retinal microglia rather than microglial cell density correlates with retinal neovascularization in the mouse model of oxygen-induced retinopathy.
Activation of retinal microglia rather than microglial cell density correlates with retinal neovascularization in the mouse model of oxygen-induced retinopathy.
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DOI：
10.1186/1742-2094-8-120
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发表时间：
2011-09-23
影响因子：
9.3
通讯作者：
Agostini HT
中科院分区：
文献类型：
--
作者：
Fischer F;Martin G;Agostini HT
在氧诱导视网膜病变(OIR)的小鼠模型中，视网膜新生血管已被广泛研究。在这里，我们研究了小胶质细胞在高氧期血管退化和缺氧期视网膜新生血管中的作用。在CX3CR1启动子标记的小胶质细胞下表达绿色荧光蛋白(GFP)的小鼠从出生后第7天(P7)到第12天一直被饲养在75%的氧气中。对不同时间点、不同视网膜位置的小胶质细胞密度进行定量。小胶质细胞的激活由分枝细胞形态向变形细胞形态的转变决定，这与GFP阳性细胞从凝集素阴性染色向凝集素阳性染色转变有关。视网膜周边区小胶质细胞密度恒定。而在中央区的深血管层，从P12到P14下降了14倍，之后又恢复。P8~P12和P16~P18中央无血管区浅层可见活化的小胶质细胞。此外，在中央区域的玻璃体层可见透明细胞，其细胞密度随时间的推移而降低。小胶质细胞密度与血管闭塞或血运重建无关。但小胶质细胞激活的时间进程表明，它们可能参与了缺氧期视网膜新生血管的形成。
Retinal neovascularization has been intensively investigated in the mouse model of oxygen-induced retinopathy (OIR). Here, we studied the contribution of microglial cells to vascular regression during the hyperoxic phase and to retinal neovascularization during the hypoxic phase. Mice expressing green fluorescent protein (GFP) under the Cx3cr1 promoter labeling microglial cells were kept in 75% oxygen from postnatal day 7 (P7) to P12. Microglial cell density was quantified at different time points and at different retinal positions in retinal flat mounts. Microglial activation was determined by the switch from ramified to amoeboid cell morphology which correlated with the switch from lectin negative to lectin positive staining of GFP positive cells. Microglial cell density was constant in the peripheral region of the retina. In the deep vascular layer of the central region, however, it declined 14 fold from P12 to P14 and recovered afterwards. Activated microglial cells were found in the superficial layer of the central avascular zone from P8 to P12 and from P16 to P18. In addition, hyalocytes were found in the vitreal layer in the central region and their cell density decreased over time. Density of microglial cells does not correlate with vascular obliteration or revascularization. But the time course of the activation of microglia indicates that they may be involved in retinal neovascularization during the hypoxic phase.
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影响因子：
3.4
作者：
Ehlken, Christoph;Martin, Gottfried;Agostini, Hansjuergen T.
通讯作者：
Agostini, Hansjuergen T.
在缺血性视网膜病变模型中，视网膜血管修复和新生血管形成不依赖于 CX3CR1 信号传导。
影响因子：
3.4
作者：
Zhao, Lian;Ma, Wenxin;Fariss, Robert N.;Wong, Wai T.
通讯作者：
Wong, Wai T.
影响因子：
4.4
作者：
Kataoka, Keiko;Nishiguchi, Koji M.;Terasaki, Hiroko
通讯作者：
Terasaki, Hiroko
影响因子：
6
作者：
Gardiner, TA;Gibson, DS;Stitt, AW
通讯作者：
Stitt, AW
影响因子：
82.9
作者：
Ishida, S;Yamashiro, K;Adamis, AP
通讯作者：
Adamis, AP