Functional polymorphisms in rhesus macaque FCGRT and β2-m.
Functional polymorphisms in rhesus macaque FCGRT and β2-m.
复制标题
恒河猴 FCGRT 和 β2-m 的功能多态性。
DOI：
10.1007/s00251-017-1022-6
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发表时间：
2018
期刊：
影响因子：
3.2
通讯作者：
Poonia,Bhawna
中科院分区：
文献类型：
--
作者：
Shubin,Zhanna;Tagaya,Yutaka;Poonia,Bhawna
恒河猴是一种重要的动物模型，用于研究测试干预措施，如抗体治疗;因此，了解影响抗体再循环的基因功能的个体间变异对于优化实验设计非常重要。新生儿Fc受体（FcRn）是由FCGRT和β2-m链组成的异源二聚体，在延长IgG的分解代谢半衰期中起着关键作用。我们研究了恒河猴FcRn的基因组多态性，并通过评估与血清IgG或β2-m水平的相关性来询问它们是否有功能。我们测试了75只动物，并报告了FcRn启动子中的VNTR多态性以及β2-m信号肽中的单核苷酸多态性的存在。VNTR次要等位基因与血清IgG水平较低相关。这种多态性可以解释动物间抗体水平的差异，并与研究疫苗诱导的抗体应答和被动免疫的恒河猴研究的有效设计相关。
Rhesus macaque is an important animal model for studies testing interventions like antibody therapeutics; as such knowledge of inter-individual variations in function of genes affecting antibody recycling is important for optimal experimental design. Neonatal Fc receptor (FcRn), a heterodimer composed of FCGRT and β2-m chains, plays critical role in extending catabolic half-life of IgG. We studied genomic polymorphisms in rhesus macaque FcRn and asked if they are functional by assessing correlations with serum IgG or β2-m levels. We tested 75 animals and report the presence of a VNTR polymorphism in promoter of FcRn as well as a single nucleotide polymorphism in the signal peptide of β2-m. A VNTR minor allele was associated with lower levels of serum IgG. This polymorphism may account for inter-animal variation in antibody levels and has relevance for effective design of rhesus macaque studies investigating vaccine-induced antibody responses and passive immunizations.