TRYPANOSOMA-CONGOLENSE - APPEARANCE AND DISTRIBUTION OF VARIABLE ANTIGEN TYPES DURING METACYCLIC DIFFERENTIATION INVITRO
TRYPANOSOMA-CONGOLENSE - APPEARANCE AND DISTRIBUTION OF VARIABLE ANTIGEN TYPES DURING METACYCLIC DIFFERENTIATION INVITRO
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DOI：
10.1017/s0031182000060170
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发表时间：
1990-02-01
期刊：
影响因子：
2.4
通讯作者：
ROSS, CA
中科院分区：
文献类型：
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作者：
PRAIN, CJ;ROSS, CA
在一种能够控制昆虫种群密度的培养系统中，研究了上胚体的分化和侵染性松材线虫的产生。培养物的扫描电子显微镜详细地显示了附着体的附着位置，显示它们以“簇”或“束”的形式附着，并具有伴生的纤维结构。在单个束内或与两个束内观察到有分裂的上胚珠。接种后第7天，用偏环型可变抗原型(M-VAT)特异性单抗进行免疫荧光抗体试验(IFAT)检测偏环型。IFAT和免疫金标记法观察到，表达M-VATS的锥体呈束状分布。使用泊松计算进行的统计分析表明，一般而言，表达单个M-VAT的元循环在整个文化中的分布是随机的。
Differentiation of epimastigotes and production of infective matacyclic forms of Trypansoma congolense were examined in a culture system which enabled manipulation of the population density of insect forms. Scanning electron microscopy of cultures revealed the attachment sites of epimastigotes in detail, showing them to be attached as ''clusters'' or ''bundles'' and having associated fibrillar structure. Dividing epimastigotes were observed either within individual bundles or in association with two bundles. Metacyclic forms were detected by an immunofluorescence antibody test (IFAT) using metacyclic variable-antigen type (M-VAT) specific monoclonal antibodies, by day 7 after seeding cultures. Trypansomes expressing M-VATs appeared singly in bundles, observed by both IFAT and an immunogold labelling method. Statistical analysis using Poisson calculations suggested that, in general, the distribution of metacyclics expressing individual M-VATs was random throughout cultures.