Decursin: A cytotoxic agent and protein kinase C activator from the root of Angelica gigas
Decursin: A cytotoxic agent and protein kinase C activator from the root of Angelica gigas
复制标题
DOI：
10.1055/s-2006-957785
复制
发表时间：
1996-02-01
期刊：
影响因子：
2.7
通讯作者：
Kim, IH
中科院分区：
文献类型：
--
作者：
Ahn, KS;Sim, WS;Kim, IH
用硅胶柱层析和制备型高效液相色谱法从当归根中分离纯化出一种具有细胞毒性的化合物。通过质谱、红外光谱、核磁共振氢谱和碳谱等方法对化合物进行结构分析，确定其为decursin，为一种吡喃香豆素类化合物，来源于白芷Angelica decursiva Fr. et Sav.体外细胞毒性试验表明，decursin对各种人癌细胞系显示出毒性活性，其中decursin的EDS约为5-16 μ g/ml。另一方面，decursin对正常成纤维细胞的细胞毒性相对较低。在体外，Decursin还激活蛋白激酶C（PKC），这表明decursin的细胞毒活性可能与蛋白激酶C的激活有关。
A cytotoxic compound was purified hem the root of Angelica gigas Nakai by silica gel chromatography and preparative HPLC. As a result of the struc ture analysis by mass, IR, H-1-NMR, and C-13-NMR spectrometry, the effective compound was identified as decursin, a pyranocoumarin characterized originally from Angelica decursiva Fr. et Sav. In vitro cytotoxicity testing showed that decursin displayed toxic activity against various human cancer cell lines, for which the EDS, of decursin was about 5-16 mu g/ml. On the other hand, decursin displayed relatively low cytotoxicity against normal fibroblasts. Decursin also activated protein kinase C (PKC) in vitro, which indicates that the cytotoxic activity of decursin may be related to the protein kinase C activation.