ELISpot for measuring human immune responses to vaccines.
ELISpot for measuring human immune responses to vaccines.
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DOI：
10.1586/erv.10.169
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发表时间：
2011-03
影响因子：
6.2
通讯作者：
Disis ML
中科院分区：
文献类型：
--
作者：
Slota M;Lim JB;Dang Y;Disis ML
酶联免疫吸附斑点（ELISpot）试验是最常用的方法之一，以测量抗原特异性T细胞在小鼠和人类。该方法受欢迎的一些主要原因是ELISpot是高度定量的，可以测量广泛的应答幅度，并且能够评估关键的细胞免疫相关活性，例如IFN-γ分泌和颗粒酶B释放。此外，ELISpot不仅适用于评价各种T细胞功能，而且适用于评价B细胞和先天免疫细胞。难怪ELISpot已经从一种研究工具发展成为一种临床检测方法。最近在各种恶性肿瘤中测试的癌症疫苗的I期和II期研究表明，ELISpot可能是预测治疗性免疫调节后临床益处的有用的生物标志物测定。本文将讨论ELISpot的最常见的应用，概述了已经进行的标准化测定和应用该方法在人体临床试验分析的努力，并描述了在开发临床级ELISpot的过程中的一些重要步骤。
The enzyme-linked immunosorbent spot (ELISpot) assay is one of the most commonly used methods to measure antigen-specific T cells in both mice and humans. Some of the primary reasons for the popularity of the method are that ELISpot is highly quantitative, can measure a broad range of magnitudes of response and is capable of assessing critical cellular immune-related activities such as IFN-γ secretion and granzyme B release. Furthermore, ELISpot is adaptable not only to the evaluation of a variety of T-cell functions, but also to B cells and innate immune cells. It is no wonder that ELISpot has evolved from a research tool to a clinical assay. Recent Phase I and II studies of cancer vaccines, tested in a variety of malignancies, have suggested that ELISpot may be a useful biomarker assay to predict clinical benefit after therapeutic immune modulation. This article will discuss the most common applications of ELISpot, overview the efforts that have been undertaken to standardize the assay and apply the method in the analysis of human clinical trials, and describe some important steps in the process of developing a clinical-grade ELISpot.
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影响因子：
5.4
作者：
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通讯作者：
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影响因子：
2.2
作者：
Disis, ML;dela Rosa, C;Maecker, HT
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作者：
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TARKOWSKI, A
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作者：
Godard, B;Gazagne, A;Tartour, E
通讯作者：
Tartour, E
对结核分枝杆菌感染的ELISPOT测试的纵向评估。
影响因子：
15.8
作者：
Hill, Philip C.;Brookes, Roger H.;Fox, Annette;Jackson-Sillah, Dolly;Jeffries, David J.;Lugos, Moses D.;Donkor, Simon A.;Adetifa, Ifedayo M.;de Jong, Bouke C.;Aiken, Alex M.;Adegbola, Richard A.;McAdam, Keith P.
通讯作者：
McAdam, Keith P.