Instability of sensory histidine kinase mRNAs in Escherichia coli
Instability of sensory histidine kinase mRNAs in Escherichia coli
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DOI：
10.1046/j.1365-2443.2003.00624.x
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发表时间：
2003-02-01
期刊：
影响因子：
2.1
通讯作者：
Ohki, R
中科院分区：
文献类型：
--
作者：
Aiso, T;Ohki, R
背景：mRNA稳定性调节是基因表达的重要机制之一。为了从大肠杆菌全基因组中鉴定出在mRNA降解过程中表达受到有效调控的基因，我们先前进行了差异显示PCR作为第一步。在筛选中，有人提出，来自组氨酸激酶基因narX和yojN的两种mRNA在双组分信号转导系统中非常不稳定。在这项研究中，我们分析了感觉激酶的mRNA的稳定性，例如arcB，barA，rcsC，narQ，narX和evgS mRNA.Results：组氨酸激酶的mRNA的细胞水平是非常低的，在LB培养基中培养在37 degreesC的野生型细胞中的mRNA被迅速降解。使用RNase E缺陷细胞的额外实验表明，mRNA在细胞中大量存在并表达延长的半衰期。同源反应调节基因arcA、rcsB、narP和narL的单顺反子转录半衰期为1.5- 3.4min。大肠杆菌中高效合成，但在野生型细胞中迅速降解。
Background: Regulating mRNA stability is one of the essential mechanisms in gene expression. In order to identify genes from Escherichia coli whole genome whose expression is effectively modulated during the process of mRNA decay, we previously performed differential display-PCR as the first step. In the screening, it was suggested that two mRNAs from the histidine kinase genes, narX and yojN, in a two-component signal transduction system, were extremely unstable. In this study we analysed the stability of sensory kinase mRNAs, e.g. arcB, barA, rcsC, narQ, narX and evgS mRNA.Results: The cellular level of the histidine kinase mRNAs was very low and the mRNAs were rapidly degraded in wild-type cells cultured at 37 degreesC in LB medium. Additional experiments using RNase E deficient cells indicated that the mRNAs existed abundantly and expressed a prolonged half-fife in the cells. Monocistronic transcripts of the cognate response regulator genes, arcA, rcsB, narP and narL have a half-fife of 1.5-3.4 min.Conclusions: mRNAs of the six histidine kinase genes in E. coli are synthesized efficiently, but rapidly degraded in wild-type cells.