Carbon nanotube/polystyrene composite electrode for microchip electrophoretic determination of rutin and quercetin in Flos Sophorae Immaturus.
Carbon nanotube/polystyrene composite electrode for microchip electrophoretic determination of rutin and quercetin in Flos Sophorae Immaturus.
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DOI：
10.1016/j.talanta.2007.05.019
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发表时间：
2007-10
期刊：
影响因子：
6.1
通讯作者：
Jing Xu;Haiying Zhang;Gang Chen
中科院分区：
文献类型：
--
作者：
Jing Xu;Haiying Zhang;Gang Chen
本文采用碳纳米管/聚苯乙烯（CNT/PS）复合电极作为微芯片毛细管电泳（CE）安培检测器，对枳实中的芦丁和槲皮素进行了测定。该复合电极是在熔融石英毛细管微通道内，通过加热原位聚合法制备的。用扫描电镜观察了电极中复合材料的表面形貌。通过对芦丁和槲皮素的分离和检测，验证了该体系的有效性。新的CNT为基础的CE检测器提供了显着降低检测电位，产生了显着增强的信号噪声特性，并表现出抗表面污染，因此增强了稳定性。它表现出长期的稳定性和重现性，峰电流的相对标准偏差小于5%（n=20），也应该找到广泛的应用在传统的CE，流动注射分析，和其他微流体分析系统。
In this report, carbon nanotube/polystyrene (CNT/PS) composite electrodes have been fabricated as sensitive amperometric detectors of microchip capillary electrophoresis (CE) for the determination of rutin and quercetin in Flos Sophorae Immaturus. The composite electrode was fabricated on the basis of the in situ polymerization of a mixture of CNT and styrene in the microchannel of a piece of fused silica capillary under heat. The surface morphologies of the composite in the electrodes were observed by using a scanning electron microscope. The performance of this unique system has been demonstrated by separating and detecting rutin and quercetin. The new CNT-based CE detector offered significantly lower detection potentials, yielded substantially enhanced signal-to-noise characteristics, and exhibited resistance to surface fouling and hence enhanced stability. It demonstrated long-term stability and reproducibility with a relative standard deviation of less than 5% for the peak current (n=20) and should also find a wide range of applications in conventional CE, flowing injection analysis, and other microfluidic analysis systems.