Bone marrow Derived Pluripotent Cells are Pericytes which Contribute to Vascularization
Bone marrow Derived Pluripotent Cells are Pericytes which Contribute to Vascularization
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骨髓来源的多能细胞是有助于血管化的周细胞
DOI：
10.1007/s12015-009-9097-6
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发表时间：
2009-12-01
影响因子：
4.8
通讯作者：
Grottkau, Brian E.
中科院分区：
文献类型：
--
作者：
Cai, Xiaoxiao;Lin, Yunfeng;Grottkau, Brian E.
周细胞对于血管形成至关重要，但周细胞的纯化和表征仍不清楚。平滑肌肌动蛋白 α (α-SMA) 是周细胞的制造者之一。本研究的目的是从骨髓中纯化α-SMA阳性细胞，并研究这些细胞的特性以及α-SMA阳性细胞与内皮细胞之间的相互作用。从α-SMA-GFP转基因小鼠中收获骨髓基质细胞，并通过FACS分选α-SMA-GFP阳性细胞。检测α-SMA-GFP阳性细胞的增殖特性和多向分化能力。然后通过将 α-SMA-GFP 阳性细胞和内皮细胞加载到胶原纤维连接蛋白凝胶上，应用 3D 培养模型来测试其血管化。结果表明，骨髓基质细胞大多是α-SMA-GFP阳性细胞，具有多能性，并且这些细胞在分化过程中表达α-SMA。 α-SMA-GFP 阳性细胞可以刺激内皮细胞形成管状结构，并随后在 3D 培养中形成坚固的血管网络。总之，骨髓来源的多能细胞是周细胞，可以促进血管形成。
Pericytes are essential to vascularization, but the purification and characterization of pericytes remain unclear. Smooth muscle actin alpha (alpha-SMA) is one maker of pericytes. The aim of this study is to purify the alpha-SMA positive cells from bone marrow and study the characteristics of these cells and the interaction between alpha-SMA positive cells and endothelial cells. The bone marrow stromal cells were harvested from alpha-SMA-GFP transgenic mice, and the alpha-SMA-GFP positive cells were sorted by FACS. The proliferative characteristics and multilineage differentiation ability of the alpha-SMA-GFP positive cells were tested. A 3-D culture model was then applied to test their vascularization by loading alpha-SMA-GFP positive cells and endothelial cells on collagen-fibronectin gel. Results demonstrated that bone marrow stromal cells are mostly alpha-SMA-GFP positive cells which are pluripotent, and these cells expressed alpha-SMA during differentiation. The alpha-SMA-GFP positive cells could stimulate the endothelial cells to form tube-like structures and subsequently robust vascular networks in 3-D culture. In conclusion, the bone marrow derived pluripotent cells are pericytes and can contribute to vascularization.