The ZooCAM, a new in-flow imaging system for fast onboard counting, sizing and classification of fish eggs and metazooplankton
The ZooCAM, a new in-flow imaging system for fast onboard counting, sizing and classification of fish eggs and metazooplankton
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DOI：
10.1016/j.pocean.2017.10.014
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发表时间：
2017-10
影响因子：
4.1
通讯作者：
F. Colas;M. Tardivel;J. Perchoc;M. Lunven;B. Forest;G. Guyader;M. Daniélou;S. L. Mestre;P. Bourriau;E. Antajan;M. Sourisseau;M. Huret;P. Petitgas;J. Romagnan
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文献类型：
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作者：
F. Colas;M. Tardivel;J. Perchoc;M. Lunven;B. Forest;G. Guyader;M. Daniélou;S. L. Mestre;P. Bourriau;E. Antajan;M. Sourisseau;M. Huret;P. Petitgas;J. Romagnan
在本文中，我们介绍了ZooCAM系统，该系统旨在对保存和活的后生浮游动物（即多细胞浮游动物）和大于300 µm ESD的鱼卵的大体积样品进行采样和分析。ZooCAM是专门为克服在PELGAS调查框架内分析浮游动物和鱼卵的困难而设计的，并提供高频数据。ZooCAM的鱼卵计数与解剖显微镜的结果相当。ZooCAM能够在采集后的几乎真实的时间内准确预测和快速船上验证阶段性鱼卵和沙丁鱼卵。与ZooScan的比较，一个更复杂的动物营养社区，提供了令人鼓舞的结果之间的协议2仪器。ZooCAM和ZooScan能够识别相似的群落，并在相同的样本上进行测试时，产生相似的浮游动物丰度，大小分布和大小谱斜率。然而，由于用于比较两种仪器的样本数量较少，这些结果需要进一步完善。ZooCAM的主要缺点在于轻微但合理地低估了丰度和大小，因此低估了个体和群落的生物量。ZooCAM已成功部署超过4年，在许多调查没有遭受任何重大故障。当与CUFES一起使用时，它提供了阶段性鱼卵和动物营养功能组的高分辨率地图。因此，ZooCAM是一个适当的工具，用于开发船上，高频率，高空间覆盖率的动物营养学和生态系统研究。
In this paper we present the ZooCAM, a system designed to digitize and analyse on board large volume samples of preserved and living metazooplankton (i.e.multicellular zooplankton) and fish eggs >300 µm ESD. The ZooCAM has been specifically designed to overcome the difficulties to analyse zooplankton and fish eggs in the framework of the PELGAS survey, and provide high frequency data. The ZooCAM fish eggs counts were comparable to those done with a dissecting microscope. The ZooCAM enabled the accurate prediction and fast on board validation of staged anchovy and sardine eggs in almost real time after collection. A comparison with the ZooScan, on a more complex zooplanktonic community, provided encouraging results on the agreement between the 2 instruments. ZooCAM and ZooScan enabled the identification of similar communities and produced similar total zooplankton abundances, size distributions, and size spectra slopes, when tested on the same samples. However these results need to be further refined due to the small number of samples used to compare the two instruments. The main ZooCAM drawback resides in a slight but sensible underestimation of abundances and sizes, and therefore individual and community biovolumes. The ZooCAM have been successfully deployed over 4 years, on numerous surveys without suffering any major failure. When used in line with the CUFES it provided high resolution maps of staged fish eggs and zooplanktonic functional groups. Hence the ZooCAM is an appropriate tool for the development of on board, high frequency, high spatial coverage zooplanktonic and ecosystemic studies.