THE HEAD INVOLUTION DEFECTIVE GENE OF DROSOPHILA-MELANOGASTER FUNCTIONS IN PROGRAMMED CELL-DEATH
THE HEAD INVOLUTION DEFECTIVE GENE OF DROSOPHILA-MELANOGASTER FUNCTIONS IN PROGRAMMED CELL-DEATH
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DOI：
10.1101/gad.9.14.1694
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发表时间：
1995-07-15
影响因子：
10.5
通讯作者：
STELLER, H
中科院分区：
文献类型：
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作者：
GRETHER, ME;ABRAMS, JM;STELLER, H
染色体区域 75C1,2 的缺失几乎阻止了果蝇胚胎中所有的程序性细胞死亡 (PCD)。我们之前在这个区间鉴定了一个基因 reaper (rpr)，它编码 PCD 的一个重要调节因子。在这里，我们报告了该区域中第二个基因的分离，即头部复旧缺陷（hid），它在 PCD 中起着类似的作用。 hid突变胚胎的细胞死亡水平降低，并且头部含有额外的细胞。我们克隆了hid基因，发现其表达足以在细胞死亡缺陷突变体中诱导PCD。 hid 基因似乎编码一种新的 410 个氨基酸蛋白质，其 mRNA 在胚胎发生细胞死亡的区域表达。果蝇视网膜中 hid 的异位表达导致眼睛消融。这种表型可以被杆状病毒的抗凋亡 p35 蛋白的表达完全抑制，表明 p35 可能在 hid 的遗传下游发挥作用。
Deletions of chromosomal region, 75C1,2 block virtually all programmed cell death (PCD) in the Drosophila embryo. We have identified a gene previously in this interval, reaper (rpr), which encodes an important regulator of PCD. Here we report the isolation of a second gene in this region, head involution defective (hid), which plays a similar role in PCD. hid mutant embryos have decreased levels of cell death and contain extra cells in the head. We have cloned the hid gene and find that its expression is sufficient to induce PCD in cell death defective mutants. The hid gene appears to encode a novel 410-amino-acid protein, and its mRNA is expressed in regions of the embryo where cell death occurs. Ectopic expression of hid in the Drosophila retina results in eye ablation. This phenotype can be suppressed completely by expression of the anti-apoptotic p35 protein from baculovirus, indicating that p35 may act genetically downstream from hid.