Priming induces functional coupling of N-formyl-methionyl-leucyl-phenylalanine receptors in equine neutrophils
Priming induces functional coupling of N-formyl-methionyl-leucyl-phenylalanine receptors in equine neutrophils
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DOI：
10.1002/jlb.63.3.380
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发表时间：
1998-03-01
影响因子：
5.5
通讯作者：
Chilvers, ER
中科院分区：
文献类型：
--
作者：
Brazil, TJ;Rossi, AG;Chilvers, ER
合成的甲酰肽fMLP被广泛用作哺乳动物嗜中性粒细胞的模型化学引诱物和促分泌素。尽管具有fMLP受体，马的中性粒细胞不产生超氧阴离子响应fMLP和有没有炎症反应时，fMLP皮内注射在马。用脂多糖（LPS）、血小板活化因子（PAF）、肿瘤坏死因子-α（TNF-α）预处理纯化的中性粒细胞，分别用光泽精依赖的化学发光（LDCL）和流式细胞术检测超氧阴离子的产生和形态变化。LPS、TNF-α和PAF预处理诱导fMLP引起的显著LDCL;同样，LPS预处理是fMLP刺激中性粒细胞极化的先决条件。然而，LPS未能诱导fMLP介导的马中性粒细胞的趋化性。这些数据表明，马嗜中性粒细胞fMLP受体是不是残留的，如以前认为的，但可以触发呼吸爆发活动和细胞极化反应后引发。
The synthetic formylpeptide fMLP is widely used as a model chemoattractant and secretagogue for mammalian neutrophils. Despite possessing fMLP receptors, equine neutrophils do not produce superoxide anions in response to fMLP and there is no inflammatory reaction in the horse when fMLP is injected intradermally. The functional capability of these receptors was investigated after pretreatment with recognized priming agents, Purified neutrophils were pretreated with lipopolysaccharide (LPS), platelet-activating factor (PAF), or tumor necrosis factor alpha (TNF-alpha) and superoxide anion generation and shape clange quantified by lucigenin-dependent chemiluminescence (LDCL) and flow cytometry, respectively. LPS, TNF-alpha, and PAF pretreatment induced significant LDCL in response to fMLP; similarly LPS pretreatment was a prerequisite for fMLP-stimulated neutrophil polarization in response to fMLP. However, LPS failed to induce fMLP-mediated chemotaxis of equine neutrophils. These data indicate that equine neutrophil fMLP receptors are not vestigial as previously thought but can trigger both respiratory burst activity and cell polarization responses after priming.