Immune activation increases susceptibility to oxidative tissue damage in Zebra Finches
Immune activation increases susceptibility to oxidative tissue damage in Zebra Finches
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DOI：
10.1111/j.1365-2435.2006.01191.x
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发表时间：
2006-12-01
影响因子：
5.2
通讯作者：
Sorci, G.
中科院分区：
文献类型：
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作者：
Bertrand, S.;Criscuolo, F.;Sorci, G.
1.先天免疫反应涉及产生高活性分子(活性氧和氮物种，ROS和RNS)。这些有毒化合物可以有效地摧毁入侵的病原体，但也可以非特异性地针对宿主细胞。如果炎症反应过度，ROS和RNS引起的组织损伤可能是严重的，有可能导致所谓的免疫病理。在本研究中，我们通过注射脂多糖(大肠杆菌细胞壁的脂多糖)的方法诱导斑纹雀的炎症反应，并以注射生理盐水(磷酸盐缓冲盐水)作为对照。分别于手术前和手术后24小时测量体重。我们还在注射后24小时采集了血样，以测量红细胞对标准化自由基攻击的抵抗力。对不同的个体重复实验两次，以确定结果的重复性。我们发现，与对照组相比，注射内毒素的鸟类在接下来的24天里失去了更多的身体质量。同样，内毒素个体更容易受到氧化组织损伤，因为他们的红细胞对自由基攻击的抵抗力比对照鸟类的红细胞更弱。这一结果表明，炎症反应与相关的细胞毒性化合物的产生可以产生副作用，可能导致对宿主组织的损害增加，因此代表了免疫病理成本。
1. The innate immune response involves the production of highly reactive molecules (reactive oxygen and nitrogen species, ROS and RNS). These toxic compounds can effectively destroy invading pathogens but can also, non-specifically, target host cells. Tissue damage caused by ROS and RNS can be substantial if the inflammatory response is overactive, potentially inducing a so-called immunopathology.2. In this study, we induced an inflammatory response in Zebra Finches by the way of a LPS injection (lipopolysaccharide of the cell wall of Escherichia coli), using as a control a group of birds injected with saline (phosphate-buffered saline). Body mass was measured both before and 24 h after the procedure. We also took a blood sample 24 h after injection, to measure the resistance of red blood cells to a standardized free radical attack. The experiment was repeated twice with different individuals to ascertain the repeatability of the results.3. We found that birds injected with LPS lost significantly more body mass during the 24 It that followed, compared with control individuals. Similarly, LPS individuals were more susceptible to oxidative tissue damage, as their red blood cells had a weaker resistance to a free-radical attack than the red blood cells of control birds.4. This result shows that the inflammatory response with the associated production of cytotoxic compounds can produce side-effects that may potentially result in increased damage to host tissues, therefore representing an immunopathology cost.