PreS Deletion Mutations of Hepatitis B Virus in Chronically Infected Patients With Simultaneous Seropositivity for Hepatitis-B Surface Antigen and Anti-HBs Antibodies
PreS Deletion Mutations of Hepatitis B Virus in Chronically Infected Patients With Simultaneous Seropositivity for Hepatitis-B Surface Antigen and Anti-HBs Antibodies
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DOI：
10.1002/jmv.21669
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发表时间：
2010-01-01
影响因子：
12.7
通讯作者：
Shen, Qian
中科院分区：
文献类型：
--
作者：
Huang, Xiangyan;Qin, Yanghua;Shen, Qian
B肝炎表面抗原（HBsAg）和抗-HBs抗体（抗-HBs）可在某些慢性B肝炎（CHB）患者中共存。本研究旨在进一步探讨这种共存与B型肝炎病毒（HBV）前S区缺失之间的关系。同时检测28例HBsAg和抗-HBs阳性患者（第1组）和28例HBsAg阳性抗-HBs阴性患者（第11组）血清。采用PCR产物直接测序法或克隆测序法测定两组患者HBV前S和S基因序列。S基因分型结果表明，所有样本HBV均为Ba或Ce基因亚型。组I中有7份样本携带HBV preS缺失突变。其中3例在preS 1的3'端有较大的缺失突变，突变型与全长野生型共存，其余4例在preS 2的5'端有缺失突变。所有突变株均为Ce基因亚型。I组仅2例标本出现G145 R/A突变。第11组中仅1份样品含有preS缺失突变。因此，可以得出结论，HBV preS缺失突变可能与CHB患者中HBsAg和抗-HBs共存有关（P值= 0.024）。抗-HBs阳性的慢性乙型肝炎患者的某些免疫反应可能选择preS缺失，这可能是免疫选择的标志物。J. Med. Virol. 82.23-31，2010年。(C)2009 Wiley-Liss，Inc.
Hepatitis B surface antigen (HBsAg) and anti-HBs antibodies (anti-HBs) may coexist in certain chronic hepatitis B (CHB) patients. This study was designed to further explore the relationship between this coexistence and hepatitis B Virus (HBV) preS deletions. Sera of 28 patients carrying both HBsAg and anti-HBs (Group 1) and those of another 28 HBsAg positive but anti-HBs negative patients (Group 11) were collected from CHB patients. Direct sequencing of polymerase chain reaction products or sequencing of clones was applied to both groups to determine sequences of HBV preS and S genes. Genotyping of the S gene indicated that all sampled HBVs were either Genosubtype Ba or Genosubtype Ce. Seven samples in Group I harbored HBV preS deletion mutations. Three of the seven samples showed large deletion mutations in 3' terminus of preS1 and co-existence of the mutant type and the full-length wild type, and the remaining four samples showed deletion mutations in 5' terminus of preS2. All mutant strains were found to be genosubtype Ce. Only two samples in Group I showed G145R/A mutation. Only one sample in Group 11 contained preS deletion mutation. It is therefore concluded that HBV preS deletion mutations are likely to be related to the coexistence of HBsAg and anti-HBs in CHB patients (P-value = 0.024). Some immune reactions may select for the preS deletion in CHB patients with anti-HBs, the possible marker for immune selection. J. Med. Virol. 82.23-31, 2010. (C) 2009 Wiley-Liss, Inc.