A localized elevation of cytosolic free calcium is associated with cytokinesis in the zebrafish embryo.
A localized elevation of cytosolic free calcium is associated with cytokinesis in the zebrafish embryo.
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DOI：
10.1083/jcb.131.6.1539
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发表时间：
1995-12
影响因子：
7.8
通讯作者：
MENG, CL
中科院分区：
文献类型：
--
作者：
CHANG, DC;MENG, CL
胞质分裂是细胞分裂的关键步骤，已知其在时间和位置上都受到精确调节。目前，胞质分裂的调控机制尚不清楚，尽管有人认为钙信号可能在这一过程中发挥重要作用。为了验证这一观点，我们将一种敏感的荧光Ca 2+指示剂引入斑马鱼胚胎，并使用共聚焦显微镜测量细胞分裂过程中细胞内游离Ca 2+浓度（[Ca 2 +]i）的时空变化。可见，胞质内[Ca ~（2+）]i的局部升高与胞质分裂密切相关。首先，我们发现，在胞质分裂过程中，游离Ca 2+的水平升高，局部正是在裂解位点。第二，游离Ca 2+的上升是非常迅速的，发生在沟收缩开始之前。这些观察结果强烈表明，胞质分裂可能是由钙信号触发的。此外，我们发现，这种与钙离子转运相关的钙信号主要来自内部的钙离子储存，而不是来自外部的游离钙离子;它可以被三磷酸肌醇（InsP 3）受体的拮抗剂阻断。这些结果表明，[Ca ~（2+）]i的局部升高是由内质网通过InsP 3调节的钙通道释放游离Ca ~（2+）引起的。
Cytokinesis, a key step in cell division, is known to be precisely regulated both in its timing and location. At present, the regulatory mechanism of cytokinesis is not well understood, although it has been suggested that calcium signaling may play an important role in this process. To test this notion, we introduced a sensitive fluorescent Ca2+ indicator into the zebrafish embryo and used confocal microscopy to measure the spatiotemporal variation of intracellular free Ca2+ concentration ([Ca2+]i) during cell cleavage. It was evident that a localized elevation of [Ca2+]i is closely associated with cytokinesis. First, we found that during cytokinesis, the level of free Ca2+ was elevated locally precisely at the cleavage site. Second, the rise of free Ca2+ was very rapid and occurred just preceding the initiation of furrow contraction. These observations strongly suggest that cytokinesis may be triggered by a calcium signal. In addition, we found that this cytokinesis-associated calcium signal arose mainly from internal stores of Ca2+ rather than from external free Ca2+; it could be blocked by the antagonist of inositol trisphosphate (InsP3) receptors. These findings suggest that the localized elevation of [Ca2+]i is caused by the release of free Ca2+ from the endoplasmic reticulum through the InsP3-regulated calcium channels.
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影响因子：
13.8
作者：
EHRLICH, BE;KAFTAN, E;BEZPROZVANNY, I
通讯作者：
BEZPROZVANNY, I
DOI：
10.1083/jcb.118.3.595
发表时间：
1992-08
期刊：
The Journal of cell biology
影响因子：
--
作者：
Satterwhite LL;Lohka MJ;Wilson KL;Scherson TY;Cisek LJ;Corden JL;Pollard TD
通讯作者：
Pollard TD
DOI：
10.1083/jcb.111.5.1905
发表时间：
1990-11
期刊：
The Journal of cell biology
影响因子：
--
作者：
Cao LG;Wang YL
通讯作者：
Wang YL
由三磷酸肌醇水平周期性变化驱动的细胞周期钙离子瞬变
影响因子：
64.8
作者：
CIAPA, B;PESANDO, D;WHITAKER, M
通讯作者：
WHITAKER, M
影响因子：
--
作者：
SANGER, JM;MITTAL, B;SANGER, JW
通讯作者：
SANGER, JW