S1 and N gene analysis of avian infectious bronchitis viruses in Taiwan
S1 and N gene analysis of avian infectious bronchitis viruses in Taiwan
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DOI：
10.1637/7186-033004r
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发表时间：
2004-09-01
期刊：
影响因子：
1.4
通讯作者：
Wang, CH
中科院分区：
文献类型：
--
作者：
Huang, YP;Lee, HC;Wang, CH
传染性支气管炎病毒(IBV)引起的疾病给养禽业造成巨大的经济损失。本研究的目的是调查台湾 IBV 的分子流行病学。选择1964年分离的IBV老毒株和1991-2003年分离的另外31株IBV进行N端S1基因分析。根据系统发育树，选择了 13 个菌株来对整个 S I 和部分核衣壳 (N) 基因进行测序。结果表明，台湾IBV株可分为两个不同的谱系，即台湾第一组和台湾第二组，其中一株是马萨诸塞株，一株是中国株。 H120与台湾株之间的S1基因没有发现重组。然而，S I 基因表现出明显高于 N 基因的分歧。由S1和N基因构建的系统发育树表明发生了基因间重组。由于大多数本地毒株都存在于台湾的聚集性地区，因此开发本地毒株的疫苗对于台湾的IBV控制是必要的。
The disease caused by infectious bronchitis virus (IBV) produces great economic loss for the poultry industry. The purpose of this study is to investigate the molecular epidemiology of IBV in Taiwan. An old IBV strain isolated in 1964 and another 31 strains isolated from 1991 to 2003 were selected for N-terminal S1 gene analysis. Based on their phylogenetic tree, 13 strains were selected for sequencing the entire S I and partial nucleocapsid (N) genes. The results indicated that Taiwanese IBV strains could be divided into two distinct lineages, Taiwan Group 1 and Taiwan Group II, with one Massachusetts strain and one Chinese strain. No recombination was found between H120 and the Taiwanese strains in the S1 gene. However, the S I gene showed a noticeably higher divergence than the N gene. The phylogenetic trees constructed from the S1 and N genes indicate that intergenic recombination has occurred. Since most local strains are in Taiwanese clusters, developing vaccines from local strains is necessary for IBV control in Taiwan.