Epigallocatechin gallate improves the quality of diabetic oocytes
Epigallocatechin gallate improves the quality of diabetic oocytes
复制标题
表没食子儿茶素没食子酸酯可改善糖尿病患者卵母细胞的质量
DOI：
10.1016/j.biopha.2023.114267
复制
发表时间：
2023-01-18
影响因子：
7.5
通讯作者：
Zhao, Lei
中科院分区：
文献类型：
--
作者：
Chao, Shuo;Li, Li -Jun;Zhao, Lei
背景：母体糖尿病会损害卵母细胞的质量和发育潜能。因此，研究如何改善糖尿病对卵母细胞质量的不良影响非常重要。表没食子儿茶素没食子酸酯（EGCG）具有多种生理活性，包括抗炎、抗氧化和抗糖尿病等。在本研究中，我们评估了EGCG在体外对糖尿病卵母细胞成熟的影响。
目的：研究EGCG在恢复糖尿病对卵母细胞质量的不良影响中的作用。
方法：通过单次注射链脲佐菌素（STZ）建立糖尿病小鼠模型。收集卵母细胞，并在添加/不添加EGCG的M16培养基中进行体外成熟培养。
结果：与对照组相比，糖尿病卵母细胞的纺锤体缺陷和染色体排列异常的频率更高，但EGCG有效地降低了纺锤体组装异常和染色体错配的卵母细胞的发生率。此外，EGCG显著缓解了糖尿病卵母细胞异常的线粒体膜电位（MMP），并且在添加EGCG时，糖尿病卵母细胞中调节线粒体融合（Mfn1和Mfn2）以及分裂（Drp1）的基因表达降低的情况得到显著改善。EGCG还降低了糖尿病卵母细胞中较高水平的活性氧（ROS），这可能是通过超氧化物歧化酶1（Sod1）和超氧化物歧化酶2（Sod2）表达增加来调节的。EGCG还可以减少糖尿病卵母细胞的DNA损伤。
结论：我们的研究结果表明，EGCG至少在一定程度上提高了糖尿病卵母细胞的质量。
Background: Maternal diabetes compromises the quality and developmental potential of oocytes. Therefore, it is important to study how to ameliorate the adverse effects of diabetes on oocyte quality. Epigallocatechin gallate (EGCG) has a variety of physiological activities, including anti-inflammatory, antioxidant, and anti-diabetes. In the present study, we evaluated the effect of EGCG on the maturation of diabetic oocytes in vitro.Objective: Investigating the role of EGCG in restoring the adverse effects of diabetes on oocyte quality. Methods: Diabetes mouse model was established by a single injection of streptozotocin (STZ). Oocytes were collected and matured in vitro with/without EGCG in M16 medium.Results: Compared with control, diabetic oocytes have a higher frequency of spindle defects and chromosome misalignment, but EGCG effectively reduces the incidence of oocytes with abnormal spindle assembly and chromosome mismatches. Moreover, the abnormal mitochondrial membrane potential (MMP) of diabetic oocytes is significantly alleviated by EGCG, and the reduced expression of genes regulating mitochondrial fusion (Mfn1 and Mfn2) and fission (Drp1) in diabetic oocytes is significantly increased while EGCG is added. EGCG also decreases the higher level of reactive oxygen species (ROS) in diabetic oocytes that may be regulated by the increased expression of superoxide dismutase 1 (Sod1) and superoxide dismutase 2 (Sod2). EGCG can also reduce the DNA damage of diabetic oocytes.Conclusions: Our results suggest that EGCG, at least partially, improve the quality of diabetic oocytes.