Bioactive nanoengineered hydrogels for bone tissue engineering: a growth-factor-free approach.
Bioactive nanoengineered hydrogels for bone tissue engineering: a growth-factor-free approach.
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DOI：
10.1021/nn507488s
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发表时间：
2015-02
期刊：
影响因子：
17.1
通讯作者：
Janet R. Xavier;T. Thakur;Prachi Desai;Manish K Jaiswal;Nicholas A. Sears;E. Cosgriff-Hernandez;R. Kaun
中科院分区：
文献类型：
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作者：
Janet R. Xavier;T. Thakur;Prachi Desai;Manish K Jaiswal;Nicholas A. Sears;E. Cosgriff-Hernandez;R. Kaun
尽管骨在创伤性损伤和骨折后具有令人印象深刻的愈合能力，但仍然存在开发促进骨不连缺损愈合的策略的显著需求。为了解决这个问题，我们开发了含有二维纳米硅酸盐的胶原基水凝胶。纳米硅酸盐是具有高度各向异性和功能性的纳米材料，与其各自的三维对应物相比，其导致与生物实体的增强的表面相互作用。与胶原基水凝胶相比，纳米硅酸盐的加入导致压缩模量沿着增加，同时孔径增加。体外评价表明，纳米复合水凝胶能够在没有任何骨诱导因子的情况下促进骨生成。碱性磷酸酶活性增加3倍，矿化基质的形成增加4倍，观察到纳米硅酸盐添加到胶原基水凝胶。总体而言，这些结果证明了纳米硅酸盐有助于骨不连缺损中骨再生的多种功能，包括增加网络刚度和孔隙率、可注射性以及在无生长因子的微环境中增强矿化基质形成。
Despite bone's impressive ability to heal after traumatic injuries and fractures, a significant need still exists for developing strategies to promote healing of nonunion defects. To address this issue, we developed collagen-based hydrogels containing two-dimensional nanosilicates. Nanosilicates are ultrathin nanomaterials with a high degree of anisotropy and functionality that results in enhanced surface interactions with biological entities compared to their respective three-dimensional counterparts. The addition of nanosilicates resulted in a 4-fold increase in compressive modulus along with an increase in pore size compared to collagen-based hydrogels. In vitro evaluation indicated that the nanocomposite hydrogels are capable of promoting osteogenesis in the absence of any osteoinductive factors. A 3-fold increase in alkaline phosphatase activity and a 4-fold increase in the formation of a mineralized matrix were observed with the addition of the nanosilicates to the collagen-based hydrogels. Overall, these results demonstrate the multiple functions of nanosilicates conducive to the regeneration of bone in nonunion defects, including increased network stiffness and porosity, injectability, and enhanced mineralized matrix formation in a growth-factor-free microenvironment.