Deletion of the Na/K-ATPase α1-subunit gene (Atp1a1) does not prevent cavitation of the preimplantation mouse embryo
Deletion of the Na/K-ATPase α1-subunit gene (Atp1a1) does not prevent cavitation of the preimplantation mouse embryo
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DOI：
10.1016/j.mod.2004.04.005
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发表时间：
2004-05-01
影响因子：
2.6
通讯作者：
Watson, AJ
中科院分区：
文献类型：
--
作者：
Barcroft, LC;Moseley, AE;Watson, AJ
Na/K-ATP酶活性的增加与空化的发生同时发生，并且与Na+泵亚基mRNA和蛋白质表达的增加相关。我们假设 Na/K-ATP 酶的 α1 同工酶是介导囊胚形成所必需的。我们使用 Atp1a1 杂合小鼠交配获得的胚胎，通过靶向破坏 Na/K-ATPase α1 亚基 (Atp1a1) 来表征小鼠的植入前发育，从而测试了这一假设。 Na/K-ATPase α1 亚基基因中存在无效突变的纯合小鼠胚胎能够经历压缩和空化。这些发现表明，在缺乏先前定位于哺乳动物滋养外胚层细胞基底外侧膜的Na+泵的主要同工酶的情况下，滋养外胚层转运机制得以维持。空化时 Na/K-ATP 酶 α 和 β 亚基的多种异构体的存在表明，催化 α 亚基的异构体之间可能存在一定程度的遗传冗余，使得囊胚形成在缺乏 α1 亚基的情况下得以进行。 (C) 2004 Elsevier Ltd. 保留所有权利。
Increases in Na/K-ATPase activity occur concurrently with the onset of cavitation and are associated with increases in Na+-pump subunit mRNA and protein expression. We have hypothesized that the alpha1-isozyme of the Na/K-ATPase is required to mediate blastocyst formation. We have tested this hypothesis by characterizing preimplantation development in mice with a targeted disruption of the Na/K-ATPase alpha1-subunit (Atp1a1) using embryos acquired from matings between Atp1a1 heterozygous mice. Mouse embryos homozygous for a null mutation in the Na/K-ATPase alpha1-subunit gene are able to undergo compaction and cavitation. These findings demonstrate that trophectoderm transport mechanisms are maintained in the absence of the predominant isozyme of the Na+-pump that has previously been localized to the basolateral membranes of mammalian trophectoderm cells. The presence of multiple isoforms of Na/K-ATPase alpha- and beta-subunits at the time of cavitation suggests that there may be a degree of genetic redundancy amongst isoforms of the catalytic alpha-subunit that allows blastocyst formation to progress in the absence of the alpha1-subunit. (C) 2004 Elsevier Ltd. All rights reserved.