Multifunctional Supramolecular Assemblies with Aggregation-Induced Emission (AIE) for Cell Line Identification, Cell Contamination Evaluation, and Cancer Cell Discrimination
Multifunctional Supramolecular Assemblies with Aggregation-Induced Emission (AIE) for Cell Line Identification, Cell Contamination Evaluation, and Cancer Cell Discrimination
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DOI：
10.1021/acsnano.0c03404
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发表时间：
2020-06-23
期刊：
影响因子：
17.1
通讯作者：
Tang, Ben Zhong
中科院分区：
文献类型：
--
作者：
Bai, Haotian;Liu, Zhiyang;Tang, Ben Zhong
细胞在生物学和医学中的重要作用毋庸置疑，但世界范围内错误鉴定和交叉污染的细胞系给相关领域带来了诸多麻烦。在此，通过四苯乙烯（TPE）衍生物和葫芦[8]脲（CB[8]）之间的主客体相互作用构建了三种超分子AIE（聚集诱导发射）纳米组装体。基于公认的AIE机理，TPE衍生物与CB组装后可以获得更强的荧光发射和更高的荧光量子产率[8]。此外，构建的超分子AIE复合物获得了充分确认的纳米结构，并表现出不同的尺寸和形状。相应地，它们产生了细胞的特征生物学特性和荧光增强。受大数据分析概念的启发，这些荧光信号通过线性判别分析进一步转化为细胞独特的指纹。我们立即在定性分析中实现了正常细胞系、两种癌细胞系和两种转移癌细胞系之间的准确鉴定。更重要的是，它很好地用于监测交叉污染细胞的评估和癌细胞的区分。作为理想超分子纳米材料的适当生物应用，该系统易于学习和应用，整个过程保持在20分钟内，无需细胞破碎、离心或洗涤步骤。
It is undoubted the important role of cells in biology and medicine, but worldwide misidentified and cross-contaminated cell lines have caused much trouble in related fields. Herein, three kinds of supramolecular AIE (aggregation-induced emission) nanoassemblies were constructed by the host-guest interaction between tetraphenylethene (TPE) derivatives and cucurbit[8]uril (CB[8]). Based on the recognized mechanism of AIE, the TPE derivatives could achieve stronger fluorescence emission and higher fluorescence quantum yield after assembling with CB[8]. Moreover, the constructed supramolecular AIE complexes obtained well-confirmed nanostructures and exhibited different sizes and shapes. Correspondingly, they generated characteristic biological properties and fluorescence enhancement of cells. Inspired by the concept of Big Data Analysis, these fluorescence signals were further transformed into a unique fingerprint of cells via linear discriminant analysis. Immediately, we realized the veracious identification between a normal cell line, two cancer cell lines, and two metastasized cancer cell lines in a qualitative analysis. More importantly, it was well used to monitor the evaluation of cross-contaminated cells and the discrimination of cancer cells. As a proper bioapplication of ideal supramolecular nanomaterials, this system was easy to learn and apply, and the whole procedure was kept to 20 min, without cell disruption, centrifugation, or washing steps.