Genome-wide analysis on protein and mRNA copy numbers in single Escherichia coli cells with single-molecule sensitivity
Genome-wide analysis on protein and mRNA copy numbers in single Escherichia coli cells with single-molecule sensitivity
复制标题
具有单分子敏感性的单个大肠杆菌细胞中蛋白质和 mRNA 拷贝数的全基因组分析
DOI：
10.1007/978-1-4939-2987-0_5
复制
发表时间：
2015
影响因子：
--
通讯作者：
Y.
中科院分区：
文献类型：
--
作者：
Taniguchi;Y.
单细胞蛋白质组学和转录组学分析是一种新兴的方法，用于提供单个细胞中基因功能的定量和全面表征。然而，这种分析经常受到检测低拷贝基因表达产物如转录因子和调节因子的灵敏度不足的阻碍。在这里，我描述了一种方法的定量全基因组分析的单细胞蛋白质和mRNA的拷贝数与单分子的灵敏度为模式生物大肠杆菌。
Single-cell proteomic and transcriptomic analysis is an emerging approach for providing quantitative and comprehensive characterization of gene functions in individual cells. This analysis, however, is often hampered by insufficient sensitivity for detecting low copy gene expression products such as transcription factors and regulators. Here I describe a method for the quantitative genome-wide analysis of single-cell protein and mRNA copy numbers with single molecule sensitivity for the model organismEscherichia coli.